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| Molecular Scale Imaging of BioSystems |
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| Objective: |
| Develop chemical and physical metrology capable of characterizing biosystems at the molecular level.
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| Description: |
| Imaging tools appropriate for characterization of biosystems are continually challenged by the need for greater spatial resolution and improved differentiation of structures of interest. Optical methods, ion beams, electron beams, and scanned probe methods all offer the ability to spatially and compositionally differentiate chemically complex system. In this project, we are developing metrology tools to characterize drug delivery systems, trace chemical impurity location/identification in implant devices, nanoscale structures for healthcare applications, taggants for enhanced contrast in organ imaging, and cancer chemotherapy agents. |
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| Area(s) of Application: |
- Health and Medical Products and Services
- Pharmaceuticals
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| Accomplishments: |
- Detection of Trace Anthropogenic Contamination in Aquatic Ecosystems using Fluorescence Excitation-Emission Matrix Spectroscopy, Environmental Technology, submitted for publication (2005). R. D. Holbrook (Surface and Microanalysis Sciences Division and P.C. DeRose (Analytical Chemistry Division)
- Cluster SIMS Depth Profiling in Polymeric Blends for Protein Drug Delivery Applications: Secondary Ion Mass Spectrometry (SIMS) methods developed at NIST provide in-depth information from polymeric blends and potentially relate the sub-surface composition to the performance characteristics in real devices.
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| Future Plans: |
- Microanalytical techniques will be explored as characterization tools for organic/inorganic interfaces relevant to emerging metrology issues in biosystems.
- Cluster SIMS: We plan to determine the 3-D molecular structure of these and other drug delivery systems (such as drug eluting stents and insulin delivery systems). We plan to monitor the diffusion and release of proteins and drugs from these systems using cluster SIMS technology. In addition to this, collaborations are being established with pharmaceutical and biomedical device manufacturing industries in order to relate the 3-D compositional structure to the performance characteristics in real devices.
- Scanned probe and optical imaging techniques will be developed with enhanced sensitivity and chemical contrast for rapid assessment of trace components of biological specimen.
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| Recent publications: |
- C.M. Mahoney, S.V. Roberson, and J.G. Gillen, “ Depth Profiling of 4-Acetamindophenol-Doped Polylactic acid) Films Using Cluster Secondary Ion Mass Spectrometry” Anal. Chem. 76, 3199-3207 (2004).
- Holbrook, R.D., Wagner, M.S., Mahoney, C.M., and Wight , S.A. , “Investigating Activated Sludge Flocs Using Microanalytical Techniques: Demonstration of ESEM and TOF-SIMS for Wasterwater Applications,” Water Environment Research (Sept/Oct 2005), in press.
- Mahoney, C.M., Gillen, J.G., and Roberson, S.V., “Dynamic SIMS Utilizing SF 5 + Polyatomic Primary Ion Beams for Drug Delivery Applications,” Applied Surface Science, 231, 174-178 (2004).
- M.S. Wagner, “Molecular depth profiling of multilayer polymer films using time-of-flight secondary ion mass spectrometry,” Anal. Chem. 77, 911-922 (2005).
- G. Gillen , C. Zeissler, C. Mahoney, A. Lindstrom, R. Fletcher, P. Chi, J. Verkouteren, D. Bright, R.T. Lareau, and M. Boldman, “Automated analysis of organic particles using cluster SIMS”, Appl. Surf. Sci. 231, 186-190 (2004).
- M.S. Wagner and G. Gillen, “Impact energy dependence of SF 5 + ion beam damage of poly(methyl methacrylate) studied by time-of-flight secondary ion mass spectrometry,” Appl. Surf. Sci. 231, 169-173 (2004).
- Hernandez R.M., Richter L, Semancik S, Stranick S, Mallouk T.E, Chemistry of Materials, 16(18), 3431-3438 (2004).
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| External Collaborators: |
- Medtronics – drug eluting stent characterization
- FDA (Center for Devices and Radiological Health)- spatially resolved molecular characterization of cardiac stents.
- Cornel University – spatially resolved mapping of boron neutron capture agents in cell culture.
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| Principal Investigator:
Greg Gillen |
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