| Metrology for Gene Expression |
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| Objective: |
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Develop methods, standards, and test data to validate the quantitative analysis of microarray measurements that monitor gene expression. |
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| Description: |
| This new area of research for NIST has been initiated very recently to develop methods, standards, and test data to validate quantitative analysis of microarray measurements that monitor gene expression. |
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| Area(s) of Application: |
- Health and Medical Products and Services
- Pharmaceuticals
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| Accomplishments: |
- A consortium with the microarray biotechnology industry has been established to facilitate the development of protocols and standards for validation of microarray results. Research is focused on the spectroscopic detection and the energetics of the hybridization process in microarrays.
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| Future Plans: |
- Preparation of a review paper on platform comparisons.
- Parametric noise modeling of publicly available data sets to establish generic performance measures.
- Study the impact of spatial artifacts in microarray images.
- Establish the ability of spike-in controls to assess and manage the variability seen in microarray measurements.
- Establish a world-class microarray performance laboratory with associated measurement capabilities (quantitative real-time PCR and other reference methods).
- Co-lead the NCCLS/CLSI project “Use of External RNA Controls in Gene Expression Measurements.”
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| Recent publications: |
- L. Wang, A.K. Gaigalas, M.B. Satterfield, M. Salit, J. Noble, “Microarray Preparation Parameters and Their Effects On The Fluorescence Properties of Spotted Oligonucleotide DNA Microarrays” , Microarrays: Methods and Protocols, submitted.
- Zhou, J., Gregurick, S.K., Krueger, S., and Schwarz, F.P. “Conformational Changes in Single-stranded DNA as a Function of Temperature by SANS”, Nucleic Acids Res., 2005, submitted.
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| External Collaborators: |
- Dr. Margaret Cam, National Institutes of Health
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| Principal Investigator: Marc Salit |
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