TPOX Variants

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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

4

207.18

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 
4 [2] 214.05 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville, TN Re-extracted observed in multiple samples    
4 [3] 213.89 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville, TN Re-extracted observed in multiple samples    
4 (<6) 214.06-214.26 ABI 3130 XL ID Marie-Beatrice Tonanny & Frederic G. Brard, RCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France re-extraction and re-amplification FTA card - saliva 2 in 154,000
<5 204.74 ABI 3730 ID, PP16HS DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3130   1 in 31,330

5

212.4

ABI 310

PR, CV

Margaret Kline

Re-amplified with two kits

 

1 in 600 samples

5 [2]

214.21

ABI 377

CO

Chad Hainley

Re-amplified

Convicted offender known

1
5 [3] 218.07 - 218.09 ABI 310 ID Andrea Verzeletti, Istituto de  Medicina Legale, Universita degli Studi di Brescia, Brescia, Italia Re-extracted and Re-amplified Paternity testing case  

5 (<6)

210.96

ABI 310

PP

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

5 (<6) [2]

210.91

ABI 3100

CO

Nicole Hahn

M U F S C

Re-amplified for confirmation

 

1

5 (<6) [3]

211.04

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen - No verification

 

2

5 (<6) [4]

 211 ABI 3100 CO

Nicole Hahn

M U F S C

     
5 (<6) [5] 211.14 ABI 3100 CO Nicole Hoffman     M U F S C Previously Seen - No verification   3
5 (<6) [6] 217.85 217.89 ABI 3130XL ID Laurent Cardin & Frederic G. Brard, IRCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France re-extraction and re-amplification FTA card - saliva 2 in 25000
7.1 227.05 ABI 310 ID Janel Smith, Phoenix Police Department Present in both exemplar and seven intimate evidence samples    
7.1 [2] 226.82 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    
7.2 227.6 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN

 

 Observed in multiple samples    

7.3

225.67

ABI 377

PS

Catherine Allor

Re-amplified and Reanalyzed

Paternity samples only

1 in 33300
7.3 [2] 228.69 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville  TN      

10.1

278.08

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

 
10.1 [2] 238.51-238.64 ABI 3130XL ID Laurent Cardin and Frederic G. Brard, IRCGN, France re-extraction and re-amplification FTA card - saliva/POP4 1/50.000

10.3

 

 

PP  & PP 2.1

MD Sate Police

Re-extracted, re-amplified, re-run and sequenced

Sample is actually 1 bp del. 157 bases from the repeat.

 

11

 

 

PP1.1 & ID

MD Sate Police

Re-extracted, re-amplified, re-run and sequenced

Sample is actually 1 bp del. 157 bases from the repeat

 

13.1

250.96

ABI 377

CO

Marie Samples

Present in both exemplar and evidence samples

 

 
>13 (14) 246.79 AI 3100 CO Nicole Hahn          M U F S C      

>13

257.48

ABI 3100

ID

Angela Withrow    M U F S C

Re-amplification

 

1 in 11,512

14

250.77

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

14 [2]

250.93

ABI 377

CO

Chad Hainley

Re-amplified

Convicted offender known

1
14 [3] 246.79 ABI 3100 CO KB                       M U F S C      
14 [4] 253.67 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    
14 [5] 253.99 ABI 3130xl ID Shannon Soltysiak, NYC OCME Dept of Forensic Biology Re-amplified    
14 (>13) 246.78 ABI 3100 CO

Nicole Hahn

M U F S C

Previously Seen – no verification

   2
14 (>13) [2] 246.95 ABI 3100 CO Nicole Hoffman                     M U F S C      

15 (>13)

297.01

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

3
15 (>13) [2] 250.86 ABI 3100 CO Nicole Hahn          M U F S C      

15

250.88

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 
15 [2] 257.78 ABI 310 ID Johnson_E USACIL Re-extracted and re-amplified Reference Blood  
15 [3] 257.74

257.78

 ABI 3100 AVANT ID Julie M. Marschner, Las Vegas Metrophoitan PD Forensic Laboratory Re-amplification of reference standard 4 casework samples with matching reference standard of victim 1 (5 times in one case)

16

261.68

ABI 3130

ID/CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

16 (>13)

300.94

ABI 310

PP

Jackie Johnson, MO State Highway Patrol Crime Lab

Re-extract and Re-amplify

Convicted Offender

1 in 33739
17 (>13) 265.69 ABI 3130xl ID Sherri Phillips, Washington State Patrol CODIS Crime Laboratory Re-extracted    

21 reported variants as of 04/27/2012

 

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

6, 8, 10

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extracted 

Convicted offender

1 in 39,000

6, 8, 10 [2]

ABI 3100

ID

Misty Marra           M U F S C

Not verified/confirmed

Alabama Department of Forensic Science Convicted Offender

1 in 7840

6, 8, 10 [3]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600
6, 8, 10 [4] ABI 3130xl ID Monica Price, WA Sate Patrol Crime Lab Re-extracted Convicted Offender  

6, 9, 10

ABI 377

CO

Kelly Solis    Texas DPS

Re-extracted 

Convicted offender

1 in 39,000

6, 9, 10 [2]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

6, 9, 10 [3]

ABI 3100

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

6=9=10

 

6, 9, 10 [4]

ABI 3100

ID

Paul Mazur

Eric County CPS Forensic Lab

Buffalo, NY

Multiple sample amplifications

Casework samples

 

6, 9, 10 [5]

ABI 3100

CO

Nicole Hahn  

M U F S C

Re-amplified x 1

 

1
6, 9, 10 [6] ABI 3130XL ID Marie-Beatrice Tonanny & Frederic G. Brard,IRCGN,National Forensic Institute of the French Gendarmerie,Rosny-sous-Bois France Re-extraction and Re-amplification FTA Card - saliva

Type 2 tri-allelic pattern

 2 in 137,000

6, 10, 11

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extracted

Convicted offender

1 in 39,000

6, 10, 11 [2]

ABI 3100

ID

Laura Kuyper

M U F S C

None

AL Dept of Forensic Sciences Convicted Offender Sample

3 in 15595

6, 10, 11 [3]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600
6, 10, 11 [4] ABI 3100 CO Nicole Hoffman       M U F S C No verification - previously seen   4

6, 10, 12

ABI 310

CO

Diane Hanson Florida Dept of Law Enforcement

Re-extracted, observed in known and question samples

 

 
6, 10, 12 [2] ABI 3130 ID Jodi Luedemann, Erie County CPS Forensic Lab, Buffalo NY Identified in both evidence and known sample (multiple sample amplifications) Casework  

7, 8, 10

ABI 310

PP  & ID

Angelo ABATI, Belgium

2 extractions and 2 reun in PP 16 and 2 runs in ID

 

1 in 1100

7, 8, 10 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

7, 9, 10

ABI 3100

CO

Neil Quigley Molecular Pathology Laboratory Network, encode Paternity Systems

Repeated DNA isolation from original buccal cells sample, and re-ran Cofiler analysis

Routine paternity test

l in about 20,000
7, 9, 10 [2] ABI 3130 ID Rebecca Wallman, Suffolk County Crime Lab, Hauppauge NY Re-amplification and re-run with COFiler Reference sample 1

7, 10, 11

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

7, 10, 11 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 9, 10

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

8, 9, 10 [2]

ABI 377

ABI 310

CO

William Stubbs WA Sate Patrol

Re-extracted and amplified

Convicted offender

 

2 in 30,000

8, 9, 10 [3]

ABI 310

ID

Mark Kalinowsk

In both questioned samples and known sample

 

1

8, 9, 10 [4]

FMBIO

PP

Dayse Silva  State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 1668

8, 9, 10 [5]

ABI 3100

ID

Angela Withrow

M U F S C

Not repeated - TPOX tri-alleles very common in Alabama

Alabama Department or Forensic Science Convicted Offender

4 in 11753

8, 9, 10 [6]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

8, 9, 10  [7]

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

8, 9, 10  [8]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 9, 10 [9]

ABI 3100 AVANT

ID

Cintia Fridman, USP,Sao Paulo, Brazil

Re-amplification

Missing Children Data Base

1
8, 9, 10 [10] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
8, 9, 10 [11] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
8, 9, 10 [12] Manual Promega CTT Laboratorio de Estudios Geneticos Aplicados - Universidad Nacional de Qualimes - Argentina Re-amplified and re-analyzed   1 in 1325
8, 9, 10 [13] ABI 310 PP Melissa Martinenza

Deleware Office of the Chief Medical Examiner, DNA Unit

 Re-extracted and re-amplified   2
8, 9, 10 [14] ABI 310 CO Hal Johnson, Illinois Sate Police Samples Re-amplification Observed in suspect standard  
8, 9, 10 [15] ABI 310 CO Marci Adkins, KY State Police Forensic Lab 2 different samples, each amplified twice A balanced triallele 8, 9, 10 @ TPOX  
8, 9, 10 [16] ABI 3730xl PP STAB VIDA, Portugal Re-extraction and re-amplify Saliva in FTA card  
8, 9, 10 [17] ABI 3730 ID, ID Plus DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3730   1 in 31,330
8, 9, 11 ABI 3100 PR, ID Chun Byung-Won, NISI, Seoul, Republic of Korea Re-extraction and re-amplification Casework sample  
8, 9, 11 [2] ABI 3100 PP Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China Re-extraction, re-amplification with PP16, confirmation with AGCU 17+1 STR Kit (AGCU ScienTech, China) Paternity Samples, Type 2 1 in 28252
8, 9, 11 [3] ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3130   1 in 31,330
8, 9, 11 [4] ABI 3130 PP16 HS, ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3130   4 in 31,330

8, 10, 11

ABI 377

CO

Chris Piwonka Texas DPS

 

Re-extraction

Convicted offender

1 in 39,000

8, 10, 11 [2]

ABI 310

CO

Patricia BencivengaFlorida Dept of Law Enforcement

Re-extracted, present in both known and question samples

Distruction/vandalisn offenses

 

8, 10, 11 [3]

ABI 310

PP  & ID

CintiaAlvesI PATIMUP, Porto Portugal

Re-extracted and re-amplified

Mozambique sample

1 in 145

8, 10, 11 [4]

ABI 3100

ID

Laura Kuyper        M U F S C

Not verified

Alabama Department of Forensic Science Convicted Offender

1 in 8400

8, 10, 11 [5]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 417

8, 10, 11 [6]

ABI 3100

ID

Angela Withrow

M U F S C

Not verified

Alabama Department of Forensic Science Convicted Offender

2 in 12,293

8, 10, 11 [7]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian Mother has genotype 9/10/11, child has 8/10/11

1 in 25154

8, 10, 11 [8]

ABI 3100

ID

Mark Kalinowski, Erie County Central Police Services Forensic Lab, NY

 

Both in questioned and known samples

1

8, 10, 11 [9]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

8, 10, 11 [10]

ABI 3100

CO

Misty Marra

M U F S C

Re-amplification

 

2 in 16,579

8, 10, 11 [11]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

4

8, 10, 11 [12]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

4

8, 10, 11 [13]

ABI 3100

CO

Angela Withrow

M U F S C

Previously documented in our lab – not repeated

 

5

8, 10, 11 [14]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously documented in our lab – not repeated

 

6

8, 10, 11 [15]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

8

8, 10, 11 [16]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

8

8, 10, 11 [17]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

10

8, 10, 11 [18]

ABI 3100

CO

Nicole Hahn 

M U F S C

Previously seen, no verification

 

11
8, 10, 11 [19] ABI 310 CO Katrina Gomez, Illinois State Police   Observed in vaginal swab F1,F2 plus victim standard  
8, 10, 11 [20] ABI 3130 ID Yue Wang, Center for Medical Genetics, Houston TX Re-extracted and re-amplified Maternity Sample 1
8, 10, 11 [21] ABI 310 PP Ashley Hayes, Anne Aruncel Co Police Crime Lab Re-extracted and Re-amplify 1 sample (blood from crime scene) 1
8, 10, 11 [22] ABI 3100 ID Matsuzaki, L. N.; Cantagalli, V. D.; Coudry, R. A; Fleury – Medicina e Saúde Re-tested with PowerPlex 16 kit Paternity samples 1 in 7199
8, 10, 11 [23] ABI 3130 PP16 Ashley Wang, Delaware OCME Re-extracted and re-amplified Convicted offender sample 2
8,10,11 [24] ABI 3130 CTTV Linda Bucket, Loma Linda University Medical Center Marrow unrelated donor and recipient with 100% engraftment; re-amplification Balanced peak heights 2
8,10,11 [25] ABI 3730xl PP STAB VIDA, Portugal Re-extraction and re-amplify Saliva in FTA card  

8, 10, 12

ABI 377

CO

Chris Piwonka Texas DPS

Re-extracted

Convicted offender

1 in 39,000

8, 10, 12 [2]

ABI 310

PP  & ID

CintiaAlves IPATIMUP, Porto Portugal

Re-extracted and re-amplified

Mozambique sample

1 in 145

8, 10, 12 [3]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 556

8, 10, 12 [4]

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 25154

8, 10, 12 [5]

ABI 3100

ID

Cantagalli,V.D.;Matsuzaki,L.N.;Coudry,R.A.;

Fleury – Medicina e Saúde

Re-tested with PowerPlex 16 kit

Bone marrow donor

1 in 7199
8, 10, 12 [6] ABI 3130 PP16 Ashley Wang, Delaware OCME Re-extracted and re-amplified Convicted offender sample 2

8, 11, 12

ABI 3100

PS & CO

Misty Marra               M U F S C

Re-amplification

West Virginia Convicted Offender

1 in 10613

8, 11, 12 [2]

Manual

Promega Silver Staining Kit

David Baumgarten

American Red Cross

Re-extraction and re-amplification

 

2 in 1350
8, 11, 12 [3] ABI 3100 PP ICMP Re-extraction and amplification Type 1 l in 11000
8, 11, 14.3 ABI 3130 and 3130xl ID Valerie Huebner, Texas Dept. of Public Safety Present in multiple samples. Buccal swab and EC fraction of vaginal swab re-amplified   3 (buccal swabs, vaginal swabs SP and EC fractions)

9, 10, 11

ABI 377

CO

Karl Morton Texas DPS

Re-extraction

offender

1 in at least 20,000

9, 10, 11 [2]

ABI 377

CO

Marie Samples

Present in both evidence and exemplar sample

 

 

9, 10, 11 [3]

ABI  310

CO

Christine Moraczewski Nebraska State Patrol Crime Lab

Re-extracted

Convicted Offender (known)

1

9, 10, 11 [4]

FMBIO

PP

Dayse Silva    State University of Rio de Janeiro

Re-extracted and re-amplified

 

Paternity samples

1 in 834

9, 10, 11 [5]

ABI 3100

 

Angela Withrow

M U F S C

None (very common in Alabama)

Alabama Division of Forensic Science Convicted Offender

3 in 13913

9, 10, 11 [6]

ABI 311

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasians. Mother has genotype 9/10/11, child has 8/10/11

1 in 25254

9, 10, 11 [7]

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

9, 10, 11 [8]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600
9, 10, 11 [9] ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
9, 10, 11, [10] ABI 3100 CO Nicole Hahn             

M U F S C

 Seen previously in our lab - No verification   4
9, 10, 11, [11] ABI 3100 CO Nicole Hoffman     

M U F S C

 Previously seen - No verification   6
9, 10, 11 [12] ABI 3130xl ID Amanda Webb, SLED Reamplification   1

9, 10, 12

ABI 377

CO

Shantel Kaster Texas DPS

Re-amplified

convicted offender

1 in at least 20,000

9, 10, 12 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600
9, 11, 12 ABI 3100 PP Sun Hongyu, Chen Wenjing, Xue Tianyu. Faculty of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China Re-extraction, re-amplification with PP16, confirmation with AGCU 17+1 STR Kit (AGCU ScienTech, China). Paternity Samples, Type 2 1 in 28252
10, 10, 11 ABI 3130 CO Garon Foster, Bexar County Criminal Investigation Laboratory, San Antonio  TX Multiple samples including known standard Tri-Allelic pattern, double dose of allele 10  

10, 11, 12

ABI 377

ABI 310

CO

William Stubbs WA Sate Patrol

Re-extracted and amplified

Convicted offender

 

1 in 30,000

10, 11, 12 [2]

ABI 3100 Avant

ID

John C. Barron, Richland County Sheriff's Dept, Columbia  SC

Re-amp and Re-run

 

1

10, 11, 12 [3]

ABI 310

PP

Brandt Cassidy DNA Solutions

Re-extract and Re-Amp

Paternity testing - African-American

1 in 1452

10, 11, 12 [4]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

18 reported tri-allelic patterns as of 04/27/2012

 

* Use the following abbreviations for amplification kit names:

 

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Last Updated: 04/27/2012