 |
Micronutrients Measurement Quality Assurance Program |
|
|
About the Program
(Background, Program
Costs)
How to
Join the Program (Enrollment form)
Related Standard Reference Materials
MMQA Workshop/Tutorial
Interlaboratory Comparison Exercises
Example of Feedback Report - - Coming!!
Related
Publications
Program Impact
Sample
Certificate of Participation
For
questions or program information, please email mmqap@nist.gov.
|
|
|
|
About
the Program
Background
For the past
20 years the National Institute of Standards and Technology
(NIST) has coordinated the Micronutrients Measurement Quality
Assurance (QA) Program for laboratories that measure vitamins
and carotenoids in human serum and plasma. Interlaboratory
comparison studies are currently directed toward the measurement
of retinol, gamma-tocopherol, alpha-tocopherol, beta-carotene,
and ascorbic acid. Laboratories also report values for delta-tocopherol,
retinyl palmitate, alpha-carotene, lycopene, beta-cryptoxanthin,
lutein, zeaxanthin, 25-hydroxyvitamin D, coenzyme Q10, vitamin
K1, and other related measurands. Frozen or freeze-dried
sera are sent to laboratories biannually (November and April)
for analysis. Results are then returned to NIST for data
evaluation and tabulation . Value-assignment of the sample
pools is based on the median of all the laboratory results,
with confirmation based on measurement at NIST using two
or more different methods. Consultation and troubleshooting
regarding methods of analysis are also provided. A micronutrient
analysis QA workshop for fat-soluble vitamin and carotenoid
analysis and for ascorbic acid in serum analysis is hosted
as well. A certificate of participation in the QA program,
which displays the laboratory's measurement performance
summary for all analytes reported, is issued at the end
of each calendar year.
|
|
|
|
|
Program
Costs (for 2004)
|
Study
|
Exercises(s)
per year
|
Annual
Participation Fee
|
|
|
(U.S.)
|
(non-U.S.)
|
| Fat-soluble
vitamins and carotenoids in serum |
2
|
$1600
|
$2000
|
| Vitamin
C in serum |
1
|
$800
|
$1000
|
|
|
|
|
|
How
to Join the Program
Enrollment
Form
If you are interested
in joining our program, please download
and print the on-line enrollment form (PDF format).
You may also obtain more information or enroll by contacting
the Micronutrients Measurement Quality Assurance Program
at the address shown below.
Micronutrients
Measurement Quality Assurance Program
NIST
100 Bureau Dr, Stop 8392
Gaithersburg, MD 20899-8392
Fax: 301-977-0685
E-mail: mmqap@nist.gov
|
|
|
|
|
Related
Standard Reference Materials (SRMs) 968c and 970
We recommend
that labs obtain Standard Reference Material (SRM) SRM
968c Fat-Soluble Vitamins, Carotenoids, and Cholesterol
in Human Serum and SRM
970 Ascorbic Acid in Human Serum at the outset of their
participation in the QA program to validate their methodology
and value assign in-house control materials. These SRMs
may be purchased from the Standard Reference Materials Program
at NIST (301-975-6776; fax: 301-948-3730; or e-mail: srminfo@nist.gov).
|
|
|
|
|
Micronutrients
Measurement Quality Assurance Workshop
This workshop
is intended for laboratories that measure vitamins and carotenoids
in human serum and plasma as part of the QA program. This
workshop provides a forum for participants to discuss analytical
methods, results of the intercomparison exercises, and other
related topics. There is no registration fee. The next workshop
has been scheduled in conjunction with the Experimental
Biology meeting at the DC Convention Center, Washington,
DC on Wednesday, April 21, 2004, 9:00am -2:30pm. For more
conference information go to: www.faseb.org/meetings/eb2004.
|
|
|
|
|
Interlaboratory
Comparison Exercises
Examples of summary
tables of noncoded data for round-robin exercises will be
available. Note that these are very large compilations of
data and that you will need to expand the image using the
"zoom-in" tool of Adobe Acrobat Reader in order
to see the information in each of these files.
CONFIDENTIALITY
STATEMENT:
It is our policy that your laboratory identification number
remains confidential. The data generated by this program
are also confidential and are provided for your use only.
Any data from other sources are included for comparison
purposes only.
Examples coming!
|
|
|
|
|
Related
Publications 
|
1
|
Brown Thomas, J., Christensen, R.G., Rieger. R., Malone,
and W. May, W.E.,Determination of Glycyrrhetinic Acid
in Human Plasma by High Performance Liquid Chromatography,
J. Chromatogr 568 (1991) 232-238. |
|
2
|
Brown Thomas, J. and Sharpless, K. E., eds., Methods
for Analysis of Cancer Chemopreventive Agents in Human
Serum; National Institute of Standards and Technology:
Gaithersburg, MD, (1995), 109-109. |
|
3
|
Brown Thomas, J.; Kline, M. C.; Schiller, S. B.; Ellerbe,
P. M.; Sniegoski, L. T.; Duewer, D. L.; Sharpless, K.
E. Certification of Fat-Soluble Vitamins, Carotenoids,
and Cholesterol in Human Serum. Fresenius' J.Anal.Chem.
356 (1996), 1-9. |
|
4
|
Brown Thomas, J.; Kline, M. C.; Duewer, D. L.; Sharpless,
K. E. The Stability of Fat-Soluble Vitamins and Carotenoids
in Human Serum. Clin.Chim.Acta , Clin.Chim.Acta , 276
(1998), 75-87. |
|
5
|
Brown Thomas. J.; Kline, M.C.; Gill, L.M.; Yen, J.H.;
Duewer, D.L.; Sniegoski, L.T.; and Sharpless, K.E. Preparation
and Value Assignment of Standard Reference Material
968c Fat-Soluble Vitamins, Carotenoids, and Cholesterol
in Human Serum, Clin. Chim. Acta., 305 (2001), 141-155. |
|
6
|
Christensen, R.G. and Malone, W., Oltipraz in Serum
by High-Performance Liquid Chromatography with Optical
Absorbance and Mass Spectrometric Detection, J. Chromatogr.
584 (1992), 207-212. |
|
7
|
Craft, N.E., Sander, L.C., and Pierson,
H.F., Separation and Relative Distribution of all-trans-$-Carotene
and its cis Isomers in beta-Carotene Preparations, J.
Micronutrient Analysis 8 (1990), 209-221. |
|
8
|
Craft, N.E. and Soares, Jr., J.H., Relative
Solubility, Stability, and Absorptivity of Lutein and
beta-Carotene in Organic Solvents, J. Agric. Food Chem.
40 (1992), 431-434. |
|
9
|
Craft, N.E., Wise, S.A., and Soares, Jr.,J.H.,
Optimization of an Isocratic High-Performance Liquid
Chromatographic Separation of Carotenoids, J.Chromatogr.
589 (1992), 171-176. |
|
10
|
Craft, N.E., Epler, K.S., Butler, T.A.,
May, W.E., and Ziegler, R.G., Evaluation of Serum Volume
Losses During Long-Term Storage, J. Res. Natl. Instit.
Stand. Technol., 98 (1993), 355. |
|
11
|
Dalluge, J.J., Nelson, B.C., Thomas, J.
B.. Welch, M.J., and Sander, L.C., Capillary Liquid
Chromatography/Electrospray Mass Spectrometry for the
Separation and Detection of Catechins in Green Tea and
Human Plasma, Rapid Communications in Mass Specrometry,
11 (1997), 1753-1756. |
|
12
|
Dalluge, J.J., Nelson, B.C., Brown Thomas,
J., Sander, L.C., Selection of Column and Gradient Elution
System for the Separation of Catechins in Green Tea
Using High-Performance Liquid Chromatography, J. Chromatogr.
A 793 (1998), 265-274. |
| 13 |
Duewer, D.L., Brown Thomas, J., Kline,
M.C., MacCrehan, W.A., Schaffer, R., Sharpless, K.E.,
May, W.E., NIST/NCI Micronutrients Measurement Quality
Assurance Program: Measurement Reproducibility, Repeatability,
Stability, and Relative Accuracy for Fat-Soluble Vitamin-Related
Compunds in Human Sera, Anal. Chem. 69 (1997), 1406-1416. |
|
14
|
Duewer, D. L.; Kline, M. C.; Sharpless, K. E.; Brown
Thomas, J. M.; Gary, K. T.; Sowell, A. L. Micronutrients
Measurement Quality Assurance Program: Helping Participants
Use Interlaboratory Comparison Exercise Results to Improve
their Long-term Measurement Performance. Analytical
Chemistry 71 (1999) 1870-1878. |
|
16
|
Duewer, D. L.; Kline, M. C.; Sharpless, K. S.; Brown
Thomas, J. NIST Micronutrients Measurement Quality Assurance
Program: Characterizing Individual Participant Measurement
Performance Over Time, Anal. Chem., 72(15) (2000), 3611-3619. |
|
|
Duewer, D. L.; Kline, M. C.; Sharpless, K. S.; Brown
Thomas, J. NIST Micronutrients Measurement Quality Assurance
Program: Characterizing the Measurement Community's
Performance Over Time.Anal. Chem. 72(17) (2000), 4163-4170 |
|
18
|
Epler, K.S., Sander, L.C., Ziegler, R.G., Wise, S.A.,
and Craft, N.E., Evaluation of Reversed-Phase Liquid
Chromatographic Columns for Recovery and Selectivity
of Selected Carotenoids, J.Chromatogr. 595 (1992), 89-101. |
|
19
|
Epler, K.S., Ziegler, R.G., Craft, N.E., Liquid Chromatographic
Method for the Determination of Carotenoids, Retinoids
and Tocopherols in Human Serum and in Food, J. Chromatogr.
619 (1993), 37-48. |
|
20
|
MacCrehan, W. A. and Schönberger, E., Reversed-Phase
HPLC Separation and Electrochemical Detection of Retinol
and its Isomers, J. Chromatogr. 417 (1987) 65-78. |
|
21
|
MacCrehan, W. A. and Schönberger, E., Determination
of Retinol, alpha-Tocopherol, and beta-Carotene in Serum
by Liquid Chromatography with Absorbance and Electrochemical
Detection. Clin. Chem., 33 (1987), 1585-1592. |
|
22
|
MacCrehan, W.A., Determination of Retinol, alpha-Tocopherol,
and beta-Carotene in Serum by Liquid Chromatography,
Met. Enz. 189 (1990), 172-181. |
|
23
|
MacCrehan, W.A., Determination of Vitamin K-1 in Serum
using Catalytic-Reduction Liquid Chromatography with
Fluorescence Detection, J. Chromatogr. B, 670 (1995),
209-217. |
|
24
|
Margolis, S. A.; Duewer, D. L. The Measurement of
Ascorbic Acid in Human Plasma and Serum: Intralaboratory
Repeatability, Interlaboratory Repeatability, Interlaboratory
Reproducibility, and Stability in Lyophilized Plasma
and Metaphosphoric Acid Preserved Plasma and Serum.
Clinical Chemistry 42 (1996), 1257-1262. |
|
25
|
Margolis, S.A. and Schapira, R.M. Liquid Chromatographic
Measurement of L-Ascorbic Acid and D-Ascorbic Acid in
Biological Samples, J. Chromatogr. B 690 (1997), 25-33. |
|
26
|
Margolis, S.A. and Park, E., Stability of Ascorbic
Acid in Solutions Stored in Autosampler Vials, Clin.
Chem., 47(8) (2001), 1463-1464. |
|
27
|
Margolis, S.A., Vangel, M., and Duewer, D.L., Certification
of SRM 970, Ascorbic Acid in Serum, and the Analysis
of Associated Interlaboratory Bias in the Measurement
Process, Clin. Chem., 49 (2003), 463-469. |
|
28
|
Nelson, B.C., Brown Thomas, J., Wise,
S.A., and Dalluge, J. The Separation of Green Tea Catechins
by Micellar Electrokinetics Capillary Chromatography,
J. Microcolumn Separation 10 (1998), 671-679. |
|
29
|
Nelson, B.C., Dalluge, J.J., and Margolis, S.A., Preliminary
Application of Liquid Chromatography-Electrospray-Ionization
Mass Spectrometry to the Detection of 5-methyltetrahydrofolic
Acid Monoglutamate in Human Plasma, J Chromatogr. B
Biomed Sci. Appl. 765(2) (2001), 141-150. |
|
30
|
Nelson, B.C., Pfeiffer, C.M., Margolis, S.A., Nelson,
C.P., Affinity Extraction Combined with Stable Isotope
Dilution LC/MS for the Determination of 5-methyltetrahydrofolate
in Human Plasma, Anal. Biochem. 313(1) (2003), 117-127. |
|
31
|
Nelson, B.C., Pfeiffer, C.M., Margolis, S.A., Nelson,
C.P., Solid-phase Extraction-electrospray Ionization
Mass Spectrometry for the Quantification of Folate in
Human Plasma or Serum, Anal. Biochem. 325(1) (2004),
41-51. |
|
32
|
Sander, L. C.; Sharpless, K. E.; Craft, N. E.; Wise,
S. A. Development of Engineered Stationary Phases for
the Separation of Carotenoid Isomers. Analytical Chemistry
66 (1994), 1667-1674. |
|
33
|
Sander, L.C., Sharpless, K.E., and Pursch,
M., C30 Stationary Phases for the Analysis of Food by
Liquid Chromatography. J. Chromatogr. A 880 (2000),
189-202. |
|
34
|
Sharpless, K. E.; Duewer, D. L. Population Distributions
and Intralaboratory Variance forFat- Soluble Vitamin-Related
Compounds in Human Serum. Analytical Chemistry 67 (1995),
4416-4422. |
|
35
|
Sharpless, K. E.; Brown Thomas, J.; Sander, L. C.;
Wise, S. A. Liquid Chromatographic Determination of
Carotenoids in Human Serum Using an Engineered C30 Stationary
and a C18 Stationary Phase, .J.Chromatogr.B 678 (1996),
187-195. |
|
36
|
Sharpless, K. E.; Schiller, S. B.; Margolis, S. A.;
Brown Thomas, J.; Iyengar, G. V.; Colbert, J. C.; Gills,
T. E.; Wise, S. A.; Tanner, J. T.; Wolf, W. R. Certification
of Nutrients in Standard Reference Material 1846: Infant
Formula. J.AOAC Int. 80 (1997), 611-621. |
|
37
|
Sharpless, K. S.; Brown Thomas, J.; Turley, C. P.;
Brewster, M. A. Vitamin A. In Laboratory Medicine: A
Scientific and Managerial Infobase; Pesce Kaplan Publishers:
Cincinnati, 1997. |
|
38
|
Sharpless, K. S.; Brown Thomas, J.; Turley, C. P.;
Brewster, M. A. Vitamin E. In Laboratory Medicine: A
Scientific and Managerial Infobase; Pesce Kaplan Publishers:
Cincinnati, 1997. |
|
39
|
Sharpless, K. E.; Welch, M. J.; Greenberg, R. R.;
Iyengar, V. I.; Colbert, J. C. Recent SRMs for Organic
and Inorganic Nutrients in Food Matrices. Fresenius'
J.Anal.Chem. 360 (1998), 456-458. |
|
40
|
Sharpless, K. E.; Arce-Osuna, M.; Brown Thomas, J.;
Gill, L. M. Value Assignment of Retinol, Retinyl Palmitate,
Tocopherol, and Carotenoid Concentrations in Standard
Reference Material 2383 Baby Food Composite. J.AOAC
Int. 82 (1999), 288-296. |
|
41
|
Sharpless, K. E.; Gill, L. M.; Margolis, S. A.; Wise,
S. A. Preparation of Standard Reference Material 2383
(Baby Food Composite) and Use of an Interlaboratory
Comparison Exercise for Value Assignment of Its Nutrient
Concentrations. J.AOAC Int. 82 (1999), 288. |
|
42
|
Sharpless, K. E.; Gill, L. M.; Margolis, S. A.; Wise,
S. A. Certification of Nutrient Concentrations in Standard
Reference Material 2383; Baby Food Composite. J.AOAC
Int., in press. |
|
43
|
Sharpless, K. E.; Gill, L. M. Value-Assignment of
Nutrient Concentrations in Five Standard Reference Materials
and Six Reference Materials. J.AOAC Int., in press. |
|
44
|
Sharpless, K. E.; Brown Thomas, J. M.; Margolis, S.
A. Liquid Chromatographic Methods for the Determination
of Vitamins in Food-Matrix Standard Reference Materials.
J. Chromatogr. A , 881 (2000) 171-181. |
|
45
|
Sharpless, K. E.; Gill, L. M. Value-Assignment of
Nutrient Concentrations in Five Standard Reference Materials
and Six Reference Materials. J.AOAC Int., 83 (2000)
413-423. |
|
46
|
Sharpless, K.E., Margolis, S.A, and Brown Thomas,
J., Determination of Vitamins in Food-Matrix Standard
Reference Materials, J. Chromatogr. A 881 (2000) 171. |
|
47
|
Sharpless, K.E., Colbert, J.C., Greenberg, R.R., Schantz,
M.M., and Welch, M.J., Recent Developments in Food-Matrix
Standard Reference Materials at NIST, Fresenius J. Anal.
Chem., 370 (2001), 275. |
|
48
|
Sharpless, K.E., Brown Thomas, J., Nelson, B.C., Phinney,
C.S., Sieber, J.R., Wood, L.J., Yen, J.H., Howell, D.W.,
Value Assignment of Nutrient Concentrations in Standard
Reference Material 2384 Baking Chocolate, J.Agric. Food
Chem. 50 (2002), 7069. |
|
49
|
Sharpless, K.E., Phinney, C.S., Wood, L.J., Yen, J.H.,
and Howell, D.W., Value Assignment of Nutrient and Aflatoxin
Concentrations in Standard Reference Material 2387 Peanut
Butter, J.Agric. Food Chem., 51 (2003) 6745. |
|
50
|
Welch, M.J., Colbert, J.C., Gill, L.M., Phinney, C.S.,
Sharpless, K.E., Sniegoskik, L.T, and Wood, L.J., "The
Certification of SRM 1546 - - Meat Homogenate, A New
Reference Material for Nutrients in a High Protein/High
Fat Matrix," Fresenius J. Anal. Chem., 370, 42
(2001). |
|
|
|
|
Program
Impact
Testimonials
The
following comments regarding the impact of the NIST Micronutrients
Measurement Quality Assurance Program have been received:
"The
addition of vitamin A to my list of analyses would have
been essentially impossible without SRM 968 and the assistance
of the capable staff at NIST. The truth was then (and still
is) that there are no other programs, no other reference
materials out there for those of us who measure these analytes...the
availability of the Micronutrients Program (SRM and round
robin samples) has enabled me to provide service to patients"...
P.
Radmacher, Research Manager
Department of Pediatrics
University of Louisville
"Obviously
the Program benefits us by providing the independent assessment
of our ability to measure antioxidant nutrients. In addition,
the fact that we are registered in the scheme and can show
independent documentary evidence of our analytical rating
helps in our application for funding to various bodies including
the Ministry of Agriculture Fisheries and Food. I would
also point out that as sub-optimum intakes of antioxidant
nutrients are increasingly implicated in many diseases including
coronary heart disease and cancer, there is a need for a
QA scheme to monitor the accuracy of results of the growing
number of epidemiological and clinical trials undertaken
in this area. As far as I am aware, no other scheme such
as the NIST Micronutrients Measurement Quality Assurance
scheme exists, certainly in not in Europe."
Dr.
G. Duthie, Group Leader
Antioxidants and DNA Damage Group
Rowett Research Institute
Scotland, UK
"We
have been participating in the Micronutrients Measurement
Quality Assurance Program for four years, and we think it
is the best quality program for vitamins in the world. Moreover,
the calculations are performed with the highest scientific
and statistical level. I do not know any other program which
proposes quality assurance for carotenoids."
Dr.
H. Faure, Chef de Service
Laboratoire de Biochimie C
Hopital de la Tronche
La Tronche, France
"Currently,
it (NIST Micronutrients Measurement QA Program) is the only
QA program available for the fat-soluble vitamins and carotenoids.
As a CAP/CLIA accredited laboratory, we are required to
participate in external QA programs when available. We have
used the data for this QA program at all of our accreditation
inspections. A QA program from NIST carries a certain reputation
that could be a benefit to any laboratory taking part in
the program."
I.
Osberg, Assistant Director
Pediatric Clinical Research Center CORE Laboratory
University of Colorado Health Sciences Center
The Children's Hospital
Denver, Colorado
U.S.
Participants
NIST's
mission is to develop and promote measurement, standards,
and technology to enhance productivity, facilitate trade,
and improve the quality of life. This summary offers examples
of some of the ways in which NIST's resources, personnel,
programs, and facilities have had a positive impact on science,
competitiveness, and health and safety in each of the 50
states.
The
Micronutrient Measurement Quality Assurance Program allow
NIST to Help Clinical and Biomedical Laboratories Improve
Measurements for Cancer Prevention Research. To see the
detailed list of the Impacts
in each of the 50 states, please go to: http://www.nist.gov/director/states/.
Measurement
Performance
Performance Examples coming!
|
|
|
|
|
Sample
Certificate of Participation (Example)
Examples coming!
|
|
|
|
