VWA Variants


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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

16.1

177.87

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

16.1 [2] 175.42 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville      
17.1 179.39 179.51 ABI 3130XL ID Marie-Beatrice Tonanny & Frederic G. Brard. IRCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France re-extraction and re-amplification FTA card - saliva 1 in 25000
18.2 156.57 ABI 3100/3130XL PP Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory Re-Extraction    

18.3

187.90

ABI 377

PS

Nicole Swinton

Re-extracted and re-amplified

 

 

18.3 [2]

157.63

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

18.3 [3]

185.99

ABI 3100

PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

18.3 [4] 185.44 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville Re-amplified    

22

171.33

FM BIO

PP

Susan Greenspoon

 

 

 

22 [2]

174.28

ABI 310

PP1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

Colon carcinoma, ATCC cell line CRL-2577; name:  RKO

 

22 [3]

176.03

ABI 310

vWA monoplex

Greg Sykes

ATCC

Re-extracted and re-amplified

Colon carcinoma, ATCC cell line CRL-2577; name:  RKO

 

22 [4]

198.86

ABI 310

PS

Kansas Bureau of Investigation

Re-extracted and re-amplified

"22" was in known blood, bone marrow sample, and semen stains

6 times in the same case (known blood, bone marrow, 4 semen stains)

23

174.16

ABI 310

PP

Jackie Johnson

MO State Hoghway Patrol

 

Re-extract and Re-amplify

Convicted Offender

1 in 32660

23 (>21)

202.52

ABI 3100

PS & ID

Misty Marra

MU F S C

Re-amplified x 2

WV convicted offender sample

1 in 12432

23 (>22) 174.13 ABI 3100 PP 16 Laura Kuyper M U F S C Amplification x 2   2

24

182.21

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

From a colon carcinoma (RKO derivative) with integrated

CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579;

name:  RKO-AS45-1

 

 

24 [2]

182.02

ABI 310

vWA monoplex

Greg Sykes

ATCC

Re-extracted and re-amplified

From a colon carcinoma (RKO derivative) with integrated

CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579;

name:  RKO-AS45-1

 

 

24 [3]

178.32

ABI 310

PP

Jackie Johnson

MO State Hoghway Patrol

 

Re-extract and Re-amplify

Convicted Offender

1 in 32660

25

186.03 (PP), 

188.41 (VWA MP)

ABI 310

PP, VWA MonoPlex

 

Greg Sykes

ATCC

Monoplex

allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6)

in PP profile, allele falls into TH01 size range

 

10 reported variants as of 04/03/08

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

10.3, 15, 18 ABI 3130 ID Barbara Frankovoch, B.Minyon Jones, Chris Larsen, University of North Texas Center for Human Identification Re-extraction, re-amplification and re-analysis This not only was an aff ladder variant but a tri-allele as well.  

11, 16, 17

ABI 310

PS

Sheree Enfinger

 Jacksonville Regional Operations Center FDLE

Re-extraction & amplification

Peak heights of 16 and 17 equal 11

 

11, 16, 17 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

12, 14, 15 ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  

12, 18, 19

ABI 377

PP

David W. Miller,WV State Police, Biochemistry Lab

Re-extracted and Re-amplified

Known blood specimen

 

14, 15, 17

ABI 310

PS

Gavin Turbett

Forensic Biology Lab
PathCentre, Perth, Western Australia

gavin.turbett@health.wa.gov.au

Re-extraction

offender

1 in at least 20,000

14, 15, 18

ABI 310

PS

Nora E. Rankin

Tucson Police Dept Crime Lab

Re-amplified

mixture

1

14, 16, 18

ABI 377

ABI 310

PS

William Stubbs

Washington State Patrol

Re-extracted and smplified

Convicted offender

1 in 30,000

14, 17, 18

ABI 310

PS

Angela Zimmerman

Marshall University FSC

Re-extraction X 2

Convicted offender

1 in 1120

14, 17, 18 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

14, 18, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

15, 16, 17

ABI 377

CO

Chris Piwonka

Texas DPS

Re-extracted

Convicted offender

1 in 49,000

15, 16, 17 (2)

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

15, 16, 20 ABI 3100 PP ICMP Re-extraction and amplification Type 1 1 in 11000
15, 17, 18 ABI 3130xl ID Robert Dorion, CODIS, Washington State Patrol Crime Lab, Seattle Re-extraction & re-amplification Verified by Technical Leader  

15, 17, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

16, 17, 18

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 31681

16, 17, 18 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

16, 17, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

17, 18, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

17, 19, 20

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

18, 19, 20

ABI 310

PP

KShepard & RRubocki

University of Nebraska

Re-extracted & Re-amplified

Peak Ht. of alleles 19 & 20 equals peak ht of 18.

Bone marrow transplant patient

1

18, 19, 20 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

23, 24, 25

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

19 reported tri-allelic patterns as of 04/03/2008

 

* Use the following abreviations for amplification kit names:

 

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