Validation of 21-SNP multiplex
Study Conducted	Description of Samples Tested	# Run	If redone now
Single Source (Concordance)	32 samples for 3 individuals	96	24
Mixture Samples	not performed		10
Mixture Ratio (male:female)	not performed
Mixture Ratio (male:male)	not performed
Sensitivity	1ng, 500pg, 250pg, 125pg, 63pg, 31pg, 16pg, 0pg x 12 dilution series	96	16
Sensitivity	Artificially degraded samples	18	16
Non-Human	dog, cat, guinea pig, ferret, horse, chicken, wolf, toad, rat, bull, cow, deer, badger, pigeon, spider monkey, otter, gorilla, chimpanzee and orang-utan, Micrococcus luteus, Escherichia coli and Clostridium perfringens	22	8
Precision (ABI 3100 and ABI 377)	used samples from single source and dilution series
Non-Probative Cases	6 cases, various crime scene samples plus reference buccals	18	18
Stutter	n/a
Peak Height Ratio	used samples from single source and dilution series
Cycling Parameters	not part of validation, part of previous work
Annealing Temperature	not part of validation, part of previous work
Reaction volume	not part of validation, part of previous work
Thermal cycler test	not part of validation, part of previous work
Male-specificity	n/a
Other: Population database samples	201 White Caucasian samples, 71 Indian Sub-continent and 86 Afro-Caribbean	358	300
Other: Reproducibility	Different sample types - blood, hair, semen, saliva, trace swabs	96	32
Other: CE injection parameters	Dilution series	8	8
	TOTAL SAMPLES EXAMINED	712	432
Submitted August 2007 by Lindsey A. Dixon (Strathclyde University) <lindsey.dixon@strath.ac.uk>
Publication: L.A. Dixon, C.M. Murray, E.J. Archer, A.E. Dobbins, P. Koumi, P. Gill (2005) Validation of a 21-locus autosomal SNP multiplex for forensic identification purposes. Forensic Sci Int. 154: 62–77