D5S818

Other Names	Chromosomal Location	GenBank Accession
D5	5q21-q31	G08446; has 11 repeat units AC008512; has 9 repeat units

Repeat: [AGAT] = GenBank top strand

Reported Primers Ref. PCR Primer Sequences

Set 1 Promega GenePrint^® PowerPlex™ 1.1

Set 2 453 5'-GGGTGATTTTCCTCTTTGGT-3'
5'-TGATTCCAATCATAGCCACA-3'

Set 3
ABI
AmpFlSTR^® Profiler Plus™

PCR Product Sizes of Observed Alleles

Allele (Repeat #) Set 1 Set 2 Set 3 Repeat Structure

Ref.

7 119 bp 133 bp 134 bp [AGAT]₇ 721

8 123 bp 137 bp 138 bp [AGAT]₈ 721

9 127 bp 141 bp 142 bp [AGAT]₉ 721

10 131 bp 145 bp 146 bp [AGAT]₁₀ 721

11 135 bp 149 bp 150 bp [AGAT]₁₁ 721

12 139 bp 153 bp 154 bp [AGAT]₁₂ 721

13 143 bp 157 bp 158 bp [AGAT]₁₃ 721

14 147 bp 161 bp 162 bp [AGAT]₁₄ 721

15 151 bp 165 bp 166 bp [AGAT]₁₅ 721

16 155 bp 169 bp 170 bp
Profiler Plus

Allelic Ladders: Commercially available from Promega and Applied Biosystems

Common Multiplexes: PowerPlex, Profiler, Profiler Plus

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Reported Primers	Ref.	PCR Primer Sequences
Set 1	Promega	GenePrint^® PowerPlex™ 1.1
Set 2	453	5'-GGGTGATTTTCCTCTTTGGT-3' 5'-TGATTCCAATCATAGCCACA-3'
Set 3	ABI	AmpFlSTR^® Profiler Plus™

Allele (Repeat #)	Set 1	Set 2	Set 3	Repeat Structure	Ref.
7	119 bp	133 bp	134 bp	[AGAT]₇	721
8	123 bp	137 bp	138 bp	[AGAT]₈	721
9	127 bp	141 bp	142 bp	[AGAT]₉	721
10	131 bp	145 bp	146 bp	[AGAT]₁₀	721
11	135 bp	149 bp	150 bp	[AGAT]₁₁	721
12	139 bp	153 bp	154 bp	[AGAT]₁₂	721
13	143 bp	157 bp	158 bp	[AGAT]₁₃	721
14	147 bp	161 bp	162 bp	[AGAT]₁₄	721
15	151 bp	165 bp	166 bp	[AGAT]₁₅	721
16	155 bp	169 bp	170 bp		Profiler Plus