CD4

Other Names
Chromosomal Location
GenBank Accession
 
12p12-pter
M86525; has 9 repeats

Repeat: [AAAAG] with some [AAAGG] = bottom strand (commonly used); [TTTTC] = GenBank top strand

*Note*: A different number of repeats results from using the top strand verses the bottom strand.
 
Reported Primers
Ref.
 PCR Primer Sequences
Set 1
3

5'-TTGGAGTCGCAAGCTGAACTAGCG-3' (CTTTT strand)

5'-CCAGGAAGTTGAGGCTGCAGTGAA-3' (AAAAG strand) 
Set 2
27, 124

5'-TTGGAGTCGCAAGCTGAACTAGC-3' (CTTTT strand)

5'-GCCTGAGTGACAGAGTGAGAACC-3' (AAAAG strand)

PCR Product Sizes of Observed Alleles
 
Allele

(Repeat #)
Set 1
Set 2
Repeat Structure 

Using bottom strand
Repeat Structure 

Using top strand
Ref.
4 134 bp 86 bp [AAAAG]4 [TTTTC]5 27, 344
5 139 bp 91 bp [AAAAG]5 [TTTTC]6 27, 344
6 144 bp 96 bp [AAAAG]6 [TTTTC]7 27
7 149 bp 101 bp [AAAAG]7 [TTTTC]8 27
8 154 bp 106 bp [AAAAG]8 [TTTTC]9 27, 344
8' 154 bp 106 bp [AAAAG]5AAAGG[AAAAG]2 [TTTTC]3CTTTC[TTTTC]5 27
9 159 bp 111 bp [AAAAG]6AAAGG[AAAAG]2 [TTTTC]3CTTTC[TTTTC]6 27
10 164 bp 116 bp [AAAAG]7AAAGG[AAAAG]2 [TTTTC]3CTTTC[TTTTC]7 27, 344
11 169 bp 121 bp [AAAAG]8AAAGG[AAAAG]2 [TTTTC]3CTTTC[TTTTC]8 27, 344
12 174 bp 126 bp     427
13 179 bp 131 bp     427
14 184 bp 136 bp     427
15 189 bp 141 bp     427

Allelic Ladders: Not commercially available at this time

Common Multiplexes:

Original Paper

CD4 Population Studies

CD4 Reference List
 
 

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