VWA Variants


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Allele Designation

Allele Size

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

14.2 168.84 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN Re-extracted    

16.1

177.87

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

16.1 [2] 175.42 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN      
17.1 179.39 179.51 ABI 3130XL ID Marie-Beatrice Tonanny & Frederic G. Brard. IRCGN-National Forensic Institute of the French Gendarmerie.Rosny-sous-Bois,France re-extraction and re-amplification FTA card - saliva 1 in 25000
18.2 156.57 ABI 3100/3130XL PP Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory Re-Extraction    
18.2 [2] 184.42 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville TN

 

Re-amplified    

18.3

187.90

ABI 377

PS

Nicole Swinton

Re-extracted and re-amplified

 

 

18.3 [2]

157.63

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

18.3 [3]

185.99

ABI 3100

PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

18.3 [4] 185.44 ABI 3100 ID Marsha Garcia, Orchid Cellmark, Nashville Re-amplified    
19.2 224.83 224.88 ABI 3130 ID Christine Cericeros, TX DPS El Paso Observed in two samples    
20.3 229.3 ABI 3130XL ID Genetica, DNA Laboratories, Inc., Cincinnati OH Inheritance (found in Father & Child)    

22

171.33

FM BIO

PP

Susan Greenspoon

 

 

 

22 [2]

174.28

ABI 310

PP1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

Colon carcinoma, ATCC cell line CRL-2577; name:  RKO

 

22 [3]

176.03

ABI 310

vWA monoplex

Greg Sykes

ATCC

Re-extracted and re-amplified

Colon carcinoma, ATCC cell line CRL-2577; name:  RKO

 

22 [4]

198.86

ABI 310

PS

Kansas Bureau of Investigation

Re-extracted and re-amplified

"22" was in known blood, bone marrow sample, and semen stains

6 times in the same case (known blood, bone marrow, 4 semen stains)

22 [5] 199.23 ABI 310 PS Janice Youngsteadt, Illinois State Police Forensic Science Center at Chicago Previously reported   1

23

174.16

ABI 310

PP

Jackie Johnson

MO State Hoghway Patrol

 

Re-extract and Re-amplify

Convicted Offender

1 in 32660

23 (>21)

202.52

ABI 3100

PS & ID

Misty Marra

M U F S C

Re-amplified x 2

WV convicted offender sample

1 in 12432

23 (>22) 174.13 ABI 3100 PP 16 Laura Kuyper M U F S C Amplification x 2   2

24

182.21

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

From a colon carcinoma (RKO derivative) with integrated

CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579;

name:  RKO-AS45-1

 

 

24 [2]

182.02

ABI 310

vWA monoplex

Greg Sykes

ATCC

Re-extracted and re-amplified

From a colon carcinoma (RKO derivative) with integrated

CMV-promoter-driven GADD45a expression vector, ATCC cell line CRL-2579;

name:  RKO-AS45-1

 

 

24 [3]

178.32

ABI 310

PP

Jackie Johnson

MO State Hoghway Patrol

 

Re-extract and Re-amplify

Convicted Offender

1 in 32660

24 [4]

206.22

ABI 3100/3130

Profiler Plus

Anthony Brown

RCMP

National Forensic Services Vancouver

Sequencing (courtesy of NIST)

 

once

25

186.03 (PP), 

188.41 (VWA MP)

ABI 310

PP, VWA MonoPlex

 

Greg Sykes

ATCC

Monoplex

allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6)

in PP profile, allele falls into TH01 size range

 

13 reported variants as of 12/28/11

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

10.3, 15, 18 ABI 3130 ID Barbara Frankovoch, B.Minyon Jones, Chris Larsen, University of North Texas Center for Human Identification Re-extraction, re-amplification and re-analysis This not only was an aff ladder variant but a tri-allele as well.  

11, 16, 17

ABI 310

PS

Sheree Enfinger

 Jacksonville Regional Operations Center FDLE

Re-extraction & amplification

Peak heights of 16 and 17 equal 11

 

11, 16, 17 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

12, 14, 15 ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  

12, 18, 19

ABI 377

PP

David W. Miller,WV State Police, Biochemistry Lab

Re-extracted and Re-amplified

Known blood specimen

 

14, 15, 16 ABI 3130 ID Oliver Stojković, Institute of Forensic Medicine, Faculty of Medicine, University of Belgrade, Serbia Re-extraction/re-amplification Routine paternity testing, balanced peak heights 1 in 4884

14, 15, 17

ABI 310

PS

Gavin Turbett

Forensic Biology Lab
PathCentre, Perth, Western Australia

gavin.turbett@health.wa.gov.au

Re-extraction

offender

1 in at least 20,000

14, 15, 18

ABI 310

PS

Nora E. Rankin

Tucson Police Dept Crime Lab

Re-amplified

mixture

1

14, 16, 18

ABI 377

ABI 310

PS

William Stubbs

Washington State Patrol

Re-extracted and smplified

Convicted offender

1 in 30,000

14, 17, 18

ABI 310

PS

Angela Zimmerman

Marshall University FSC

Re-extraction X 2

Convicted offender

1 in 1120

14, 17, 18 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

14, 17, 18 [3] ABI 3730 ID DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-analyzed with ABI 3730   1 in 31,330

14, 18, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

15, 16, 17

ABI 377

CO

Chris Piwonka

Texas DPS

Re-extracted

Convicted offender

1 in 49,000

15, 16, 17 (2)

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

15, 16, 20 ABI 3100 PP ICMP Re-extraction and amplification Type 1 1 in 11000
15, 17, 18 ABI 3130xl ID Robert Dorion, CODIS, Washington State Patrol Crime Lab, Seattle Re-extraction & re-amplification Verified by Technical Leader  

15, 17, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

15, 19, 20 ABI 310 PR Jennifer Dohring, Michigan State Police-Northville Crime Lab Re-extraction and re-amp Lab employee profile 2

16, 17, 18

ABI 377

In-house multiplex

Martin Whittle, Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routine parentage testing, Brazilian caucasian

1 in 31681

16, 17, 18 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

16, 17, 18 [3] ABI 3130xl PS Sorenson Forensics re-amplified Mummified toe found in parking lot. 16 and 18 are balanced (91.5%. 17 allele is 26% of 18 allele  

16, 17, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

16, 18, 19 ABI 3500 ID Plus Bill Gartside, Wyoming State Crime Laboratory Re-extracted and re-amplified   Type (1) 16 = 18+19

17, 18, 19

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

17, 18, 19 [2] ABI 3130xl ID Robert Dorion, Washington State Patrol Re-extraction Convicted Offender  
17, 18, 20 ABI 3130xl ID Sherri Phillips, Washington State Police CODIS Crime Laboratory Re-extracted    

17, 19, 20

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

17, 19, 20 [2] ABI 3730 PS Elizabeth H. Field, Iowa Regional Histocompatability & Immunogenetics Laboratory, Iowa City   Iowa We repeated the original sample and ran a separate sample obtained at a different time from the same individual The sum of the peak heights of alleles 19 and 20 is approximately equal to the peak height of allele 17 1

18, 19, 20

ABI 310

PP

KShepard & RRubocki

University of Nebraska

Re-extracted & Re-amplified

Peak Ht. of alleles 19 & 20 equals peak ht of 18.

Bone marrow transplant patient

1

18, 19, 20 [2]

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

18, 19, 20 [3] ABI 3130xl ID Robert Dorion, Washington State Patrol Re-extraction Convicted Offender  
18, 19, 20 [4] ABI 3730 ID, ID Plus DNA Forensic Division, Supreme Prosecutor's Office, South Korea Re-amplified and re-analyzed with ABI 3730   1 in 31,330

23, 24, 25

ABI 310

PP

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

23  reported tri-allelic patterns as of 12/28/2011

 

* Use the following abreviations for amplification kit names:

 

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