D16S539 Variants


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Allele Designation

Allele Size 

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

4

257.74

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 69600

6

237.30

ABI 377

CO

Nicole Swinton

Re-extracted and reamplified

 

 

6 [2]

233.11

ABI 310

PS,CO

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

6 [3]

233.44

ABI 310

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 

6 [4]

264.8

ABI 3130

PP 16

Jason Hodges, WV State Police Crime Lab

Re-amplified

Convicted Offender

 

6 [5]

256.03

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

7

237.50

ABI 310

CO

Russell Vossbrink

Observed in 1 k and 3 q's

Casework

 

7 [2]

269.98

ABI 3100

PP

Cory Formea, IL State Police DNA Indexing Lab

Re-amplification

Convicted Offender Sample

1

7 [3]

260.10

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

8.3

267.94

ABI 310

ID

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

8.3 [2]

277.45

ABI 310

PP16

Angelo Abati, Institut de medecine legale de  LIEGE Belgium

3 extractions, 3 PCR in Identifilier and 3 PCR in PP16 and confirmation with the father profile which has the same allele

 

1 in 800 convicted offenders (+ the father)

9.3

248.29

ABI 310

CO

MO State Highway Patrol

Re-extracted and re-amplified

Convicted Offender

1 in 4514 to date

9.3 [2]

271.71

ABI 310

ID

Angelo ABATI,

Belgium

3 times in ID

 

1 in 1100

9.3 [3]

281.26

ABI 310

PP 16

Angelo ABATI,

Belgium

3 times in PP16

 

1 in 1100

9.3 [4]

271.37

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

9.3 [5]

248.02

ABI 3100

PS

Angela Withrow        M U F S C

 

 

 

10.1

283.01

ABI 3130XL

PP

Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory

Confirmed with multiple samples

 

 

10.3

275.07

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Re-injected

 

 

10.3 [2]

275.07

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Observed in multiple samples, re-injected

 

 

10.3 [3]

274.9

ABI 3100

ID

Marsh Garcia, Orchid Cellmark, Nashville  TN

Observed in multiple samples, re-injected

 

 

11.1

287.01

ABI 3100

PP

Jaclyn Garfinkle, Illinois State Police, DNA Indexing Laboratory

Re-Extraction

 

 

11.3

256.27

ABI 310

CO

David Young

Texas DPS

Multiple amplifications

Evidence samples

1 in 7500 to date

11.3 [2]

279.13

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

3 in 12115

11.3 [3]

288.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

11.3 [4]

279.25

ABI 3100

ID

Paul Mazur

Erie County CPS Forensic Lab

Buffalo, NY

Multiple amplifications

Casework sample

 

11.3 [5]

278.96

ABI 3100

ID

Angela Withrow         M U F S C

 

 

 

11.3 [6]

278.96

ABI 3100

ID

Angela Withrow         M U F S C

 

 

 

12.1

258.39

ABI 310

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

12.1 [2]

293.04

ABI 310

PP 1.2

Greg Sykes

 ATCC

Re-extracted and Re-amplified

:  From a colon carcinoma with integrated CMV-promoter-driven GADD45a

expression vector, ATCC cell line CRL-2579; name:  RKO-AS45-1

 

12.1 [3]

290.85

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

12.1 [4]

290.98

ABI 3100

PP

ICMP

Re-extraction and amplification or observed intergenerational inheritance

Blood reference

2 in 11000

12.1 [5]

281.42

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

 

 

 

12.2

263.17

ABI 377

CO

Kelly Solis

Texas DPS

Re-amplified

Convicted offender

1 in 35000

12.3

283.46

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-amplified

 

 

13.1

297.18 (POP-4)

299.84 (POP-6)

ABI 310

PP

Greg Sykes

ATCC

Re-amplified three times and run using high resolution POP-6 polymer

allele discovered in a colon carcinoma cell line transfected with HPV E6 inserted in pCMV.3 (name designation: RKO-E6)

 

13.2

286.15

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

 

Re-injected

 

 

13.3

267.93

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

13.3 [2]

287.18

ABI 3100

ID

Angela Withrow

M U F S C

Re-amplified

Alabama Depart of Forensic Sciences Convicted Offender

2 in 12,812

13.3 [3]

296.97

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 33739

13.3 [4]

287.65 +/-0.18

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

4 in 11500

13.3 [5]

264.47

ABI 3100

CO

Angela Withrow    M U F S C

Previously seen - not verified

 

3

13.3 [6]

264.34

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen – no verification

 

4

13.3 [7]

264.47

ABI 3100

CO

Nicole Hahn

M U F S C

Previously Seen – no verification

 

5

14.1

308.45

ABI 3100

PPX16

Marsha Garcia, Orchid Cellmark, Nashville  TN

Re-amplified

 

 

14.2

290.27

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville  TN

re-injected

 

 

14.3

272.02

ABI 337

CO

Nicole Swinton

Re-extracted and re-amplified

 

 

16

273.57

ABI 310

CO

Carolyn Booker

Reproduced in blood, saliva, hair, and fingernail samples

Discovered during validation testing

One individual

16 [2]

273.81±0.10

ABI 3100

CO

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

2 in 3750

16 [3]

306.10

ABI 3100

PP

ICMP

Re-extraction and amplification or observed intergenerational inheritance

Blood reference

1 in 11000

16 [4]

296.29

ABI 3100

ID

Marsha Garcia, Orchid Cellmark, Nashville

Re-extracted

 

 

16 [5]

273.56 (average of two injections)

ABI 310

CO

Andrew Garinger, Illinois State Police

 

Observed in both victim standard and sample F1.

 

16 (>15)

305.96

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and Re-amplify

Convicted Offender

1 in 32660

17 277 ABI 3130 SGM+ Rivka Adelman, Israely Police Observed in 4 PCR of 3 items and 1 Reference (the same allele size) Casework  

21 reported variants as of 3/16/2009

 

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

7, 11, 12

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

9, 11, 12

ABI 3100

ID

Angela Withrow

 M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 15144

10, 12, 13

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

10, 12, 14.2

ABI 3130xl

 

Robert Dorion, Washington State Patrol Crime Laboratory

 

 

 

11, 12, 13

ABI 3100

ID

Angela Withrow     M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender Sample

1 in 14767

11, 12, 13, [2]

ABI 310

PP 16

Anthony J. Tambasco, Mansfield, Ohio, Police Lab

Re-injection

Two seperate crime scenes and suspect standard

1

11, 13, 14

ABI 3100

ID

Angela Withrow     M U FS  C

Two extractions/Three amps

 

1 in 12812

11, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

12, 13, 14

ABI 377

CO

Karl Morton

Texas DPS

 

Re-extracted 

Convicted offender

1 in 39,000

12, 13, 14 [2]

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

12, 14, 15 ABI 3130 ID Kristen Homich, Erie County Central Police Services
Forensic Laboratory, Buffalo  NY
Re-amplification and electrophoresis    

8 reported tri-allelic patterns as of 10/26/2009

 

* Use the following abbreviations for amplification kit names:

 

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