Lili Wang

NIST Building 227, Room A215
100 Bureau Drive
Gaithersburg, MD 20899-8312 USA
Phone: 301-975-2447
Email: lili.wang@nist.gov

 

Education

  • Ph.D. Chemistry, DOE Radiation Laboratory, University of Notre Dame, Notre Dame, IN, 1995

Current Research

  • Fluorescence Quantification
  • DNA Microarrays
  • Flow Cytometry
  • Quantitative Real-Time PCR
  • DNA and RNA Quantitation

 


Research activities

  • Research on fundamentals of fluorescence measurements pertinent to gene expression technologies: investigate spectral properties of immobilized labeled targets, labeled oligo probes and labeled cDNA probes hybridized to targets on various types of glass substrates in DNA microarrays; Examine consistency of microarray measurements by probing different regions of the same genes.

  • Characterize bacterial spore growth and storage conditions using flow cytometry; Develop quantitation methodologies for multicolor flow cytometry; Detection of simulants of biological threats using liquid suspension arrays; Quantify CD20, a B-cell marker, for B-cell Chronic Lymphocytic Leukemia.

  • Assess the performance of three major probe types, TaqMan, molecular beacon, and shared stem molecular beacon, in quantitive real-time PCR; Research on alternative methods of DNA and RNA quantitation using fluorescence signal amplification in the absence of PCR.

  • Research on fluorescence properties of phycoerythrin and silicon nanoparticle conjugated biomolecules, and on fluorescence measurement issues associated with 96-well plates.


Representative Publications

  • “Fluorescent Nanometer Microsphere Labels for Antibodies: Detection in Suspension Arrays”, L. Wang, K.D. Cole, A.K. Gaigalas, and Yu-Zhong Zhang. Bioconjugate Chemistry, 16, 194-199 ( 2005).

  • “Optical Properties of Alexa 488 and Cy5 Immobilized on a Glass Surface”, L. Wang, A.K. Gaigalas, and V. Reipa. Biotechniques, 38, 127-133 (2005).

  • “The Effect of Overhanging Nucleotides on Fluorescence Properties of Hybridizing Oligonucleotides Labeled with Alexa 488 and FAM Fluorophores”, J.E. Noble, L. Wang, K.D. Cole, and A.K. Gaigalas. Biophysical Chemistry, 113, 255-263 (2005).

  • “Silicon Nanoparticles as a Luminescent Label to DNA”, L. Wang, V. Reipa, and J. Blasic. Bioconjugate Chemistry, 15(2) 409-412 (2004).

  • “Effect of Target and Probe Concentrations on Hybridization in DNA Microarrays”, L. Wang, A.K. Gaigalas, Y. Zong, S. Zhang, J. Shi, and Y. Wang. Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II. Nicolau, D.V. and Raghavachari, R. (Editors), 129-139 (2004)

  • “Preventing Photodegradation of Fluorescein Using N-Propyl Gallate”, A.K. Gaigalas, L. Wang, and K.D. Cole. J. Phys. Chem. A 108, 4378-4384 (2004).

  • "Quantification of EGFP Expression on Molt-4 T Cells Using Calibration Standards", Y. Gerena-López, J. Nolan, L. Wang, A.K. Gaigalas, A. Schwartz, and E. Fernández-Repollet, Cytometry Part A, 60A, 21-28 (2004).

  • "Fluorescence Resonance Energy Transfer Between a Donor-Acceptor Pair on Two Oligonucleotides Hybridized Adjacently to a DNA Template", L. Wang, A.K. Gaigalas, J. Blasic, M.J. Holden, D.T. Gallagher, and R. Pires. Biopolymer (Biospectroscopy) 72, 401-412 (2003).

  • "Quantitating Fluorescence Intensity from Fluorophores: Practical Use of MESF Values", L Wang, AK Gaigalas, F Abbasi, GE Marti, RF Vogt,  and A Schwartz, Journal of Research of the National Institute of Standards and Technology 107, 339-353 (2002).