D7S820 Variants

To contribute to this table, click here.  

*Note: The commonly observed x.3 and x.1 alleles are likely the result of a variation in the number of T's found in a poly(T) stretch 13 bases downstream of the core GATA repeat. "On-ladder" alleles contain 9Ts while the x.3 alleles contain 8Ts and the x.1 alleles contain 10Ts (see STR_Ref # 1412; Egyed, B. et al. Forensic Sci. Int. 2000, 113, 25-27).

Allele Designation

Allele Size 

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

5

255.55

ABI 377

PS, CO

Nicole Swinton

Re-extracted and Reamplified

 

 

5.2

257.78

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in 33300

5.2 (<6)

210.25

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

6.2

257.26

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

6.3

262.47

ABI 377

PS, CO

Shantel Kaster

Re-amplified

Convicted offernder known

1 in 4500

6.3 [2]

259.13

ABI 310

PS,CO

Nora Rankin

Tucson PDCL

2  kits (Profiler Plus and Cofiler) on Known samples

 

1

6.3 [3]

257.93

ABI 3100

ID

Angela Withrow

M U F S C

Re-amp x 1

AL Dept  of Forensic Science Convicted Offender

7 in 16334

6.3 [4]

257.50

ABIO 3100 Avant

ID

Stefania Turrina, Dept of Medicine & Public Health, University of Verona – Italy

Re-extract and re-amplofy

 

1

6.3 [5]

258.60

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

6.3 [6]

258.82 & 258.86

ABI 3100

PS & CO

Misty Marra

M U F S C

Re-amplified

 

8 in 16,579

6.3 [7]

259

ABI 3100

CO/PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

 
6.3 [8] 258.38 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  
6.3 [9] 258.2 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  
6.3 [10] 258.90 & 258.96

 

 ABI 3100 PS & CO Nicole Hahn     M U F S C Previously seen - no verification 18182 10
6.3 [11] 214.15 ABI 3100 PP 16 Laura Kuyper   M U F S C Re-amplification x 1 BC 6961 1l
6.3 [12] 213.96 ABI 3100 PP 16 Laura Kuyper   M U F S C amplification x 2 BC 7216 1l

7.1

217.83

ABI 310

PP 1.2

Greg Sykesand Robyn Hedges

ATCC

Re-extracted and re-amplified

adenocarcinoma, ATCC cell line HTB-111; name:  AN3 CA

 

7.1 [2]

259.84

ABI 3100

ID

Angela Withrow

M U F S C

 

Re-amplified

 

1in 11,172

7.3

219.63

ABI 310

PP 1.2

Robyn Hedges

ATCC

Re-extracted and re-amplified

colorectal carcinoma (cecum), ATCC cell line CRL-2159; name:  LS411N

 

7.3 [2]

263.15

ABI 310

PS, CO

Henry Hollyday

Reamplified with two different kits

 

 

7.3 [3]

266.36

ABI 377

PS

Chad Hainley

Reamplified

Convicted offender known

1

7.3 [4]

218.95

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

7.3 [5]

261.97

ABI 3100

ID

Angela Withrow

M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender sample

3 in 15763
7.3 [6] 262.26 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  
7.3 [7] 263.14 ABI 310 PS/CO Ryan Paulsen, Illinois State Police Forensic Science CEnter at Chicago Multiple samples, kit overlap Victim standard and unknown sample  

8.1

268.27

ABI 377

PS, CO

Nicole Swinton

Re-extracted and Re-amplified

 

 

8.1 [2]

221.28

AIi 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

8.2

266.48

ABI 310

PS

Jeff Sailus

 

 

 

8.3

224.92

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

primary metaplastic carcinoma (mammary gland), ATCC cell line

CRL-2330; name:  HCC1569

 

8.3 [2]

270.34

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in 33300

8.3 [3]

223.71

ABI 310

PP1.2

Greg Sykes

ATCC Manassas  VA

Re-extracted and re-amplified

ATCC cell line CRL-2264;name: CEM/C2; from a camptothecin (CPT) resistant derivative of the human T cell leukemia cell line CCRF-CEM (parental ATCC cell line CCL-119)

 

 

8.3 [4]

267.98

ABI 3100-Avant

ID

Forensic DNA Institute at Guangdong Maternal & Children Hospital, P.R. China

Re-extracted DNA, re-amplified and re-analysis

 

1 in 170
8.3 [5] 266.11 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  

9.1

272.6

ABI 310

PR, MP

Margaret Kline

Reamplified with two kits.

 

1 in 600 samples

9.1 [2]

226.97

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

prostate carcinoma-from same patient as LNCaP clone FGC, ATCC cell line CRL-10995; name:  LNCaP-FGC-10

 

9.1 [3]

227.01

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

prostate carcinoma (metastatic site:  left supraclavicular lymph

node), ATCC cell line CRL-1740; name:  LNCaP clone FGC [also appears as

LNCaP.FGC]

 

9.1 [4]

227.8

FM BIO

PP

Susan Greenspoon

Reextracted,Reamplified,Re-electrophoresed on 43 cm gel

 

 

9.1 [5]

269.6

ABI 310

PS, CO

Russell Vossbrink

Re-extracted from kit overlap

 

1 in 200 Hispanic samples

9.1  [6]

269.39- 

269.55

ABI 310

PS, CO

Robin Rothove

Re-extracted and analyzed by another Criminalist

 

4

9.1 [7]

272.34

ABI 377

PS, CO

Robert Baumann

Amplified separately using COfiler and Profiler Plus kits; same extract used

 

1

9.1 [8]

DMRI 231.0

PP16 213.5

MegaBACE

1000

 

PP16

Rita Young & Eric Yap

Defence Medical Research Institute, Singapore

 

Used in-house STR panel, confirmed using PP16

 

3 in 368 Chinese

1 in 364 alleles for Malay

9.1 [9]

226.85

ABI 3100

PP 16

Marian,Catalin Nucleic Acids Lab,Biochemistry Dept "Victor Babes" U of Med & Pharm P/ta.Eftimie Murgu,No.2

Re-extracted and Re-amplified

 

1 in 200 Romanian Samples

9.1 [10}

267.92

ABI 3100

ID

Angela Withwow

M U F S C

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender sample

3 in 14,544

9.1 [11]

224.98

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 33739

9.1 [12]

268.27 +/- 0.25

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

5 in 11500

9.1 [13]

 

ABI 310

Pro+ & CO

Dan Merena. Arizona DPS

 

 

 

9.1 [14]

268.92

ABI 3100

PS/CO

Nicole Hahn, Marshall University

Previously seen – no verification

 

4

9.1 [15]

269.17

ABI 3100

PS/CO

Nicole Hahn, Marshall University

Previously seen – no verification

 

5
9.1 [16] 268.38 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  
9.1 [17] 268.36 ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  
9.1 [18] 268.05 ABI 310 ID King_C USACIL Re-extracted and re-amplified Reference Blood  

9.2

270.1

ABI 310

PS, CO

Kathy Sartor

Observed with kit overlap

CSO sample

1 in 600

9.2 [2]

228.08

ABI 310

PP 1.2

Greg Sykes

ATCC

 

acute T cell leukemia (T lymphocyte), derived from A3 cells treated

with to the frameshifting mutagen, ICR-191, ATCC cell line CRL-2571; name:

I 9.2.  Compared to A3, I 9.2 has an identical PP profile except it has two

allelic drop-outs and A3 has D7S820 allele 10 instead of the 9.2.

 

9.3

227.63

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

malignant melanoma, ATCC cell line HTB-72; name:  SK-MEL-28

 

9.3 [2]

227.71

ABI 310

PP 1.2

Robyn Hedges

ATCC

Re-extracted and re-amplified

colorectal carcinoma (cecum), ATCC cell line CRL-2159; name:  LS411N

 

9.3 [3]

227.51

ABI 310

PP 1.2

Robyn Hedges

ATCC

Re-extracted and re-amplified

colorectal adenocarcinoma, (ATCC cell line CL-187); name:  LS 180

 

9.3 [4]

228.84

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

colorectal adenocarcinoma (metastatic site:  left supraclavicular

region), ATCC cell line CCL-229; name:  LoVo

 

9.3 [5]

229.07

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

submitted as an ovarian adenocarcinoma, name:  MDAH-2774.  This cell

line material appears to be a mixture-it has six PP loci showing at least a

trisomy

 

9.3 [6]

271.43

ABI 310

PS, CO

Russell Vossbrink

Re-extracted from kit overlap

 

3 in 200 Hispanics

9.3 [7]

274.49

ABI 310

PR, MP

Margaret Kline

Re-amplified and verified on a gel

 

 

9.3 [8]

274.17

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in 6600

9.3 [9]

269.7

ABI 3100

ID

John C. Barron, Richland County Sheriff's Dept, Columbia  SC

Re-amplification

 

 

9.3 [10]

270.98

ABI 3100

PS & CO

Misty Marra

M U F S C

Amplification x 2

WV Convicted Offender sample

5 in 12432

9.3 [11]

270.09

ABI 310

ID

Christine Ceniceros TX DPS El Paso

Various extractions and injections

 

 

9.3 [12]

270.26

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500

9.3 [13]

271.18±0.58

ABI 3100

CO/PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

3 in 3750
9.3 [14] 270.39 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  

10.1

276.22

ABI 310

PR, MP

Margaret Kline

Re-amplified and verified on a gel

 

 

10.1 [2]

230.06

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

:  malignant papillary serous adenocarcinoma (ovarian, metastatic site:

ascites), ATCC cell line CRL-11732; name:  OV-90

 

10.1 [3]

228.39

273.08

273.16

ABI 310

PP

PS

CO

Michael Haase

Wisconsin State Crime Lab - Milwaukee

3 separate amplifications using kits from two manufacturers

African American sample

1

10.1 [4]

276.10

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in1959

10.1 [5]

273.12

ABI 3100 AVANT

CO & PS

Andrea Gemson, Tucson PD Crime Lab

Re-amplified and Re-analyzed, also kit overlap

Same individual has an OLA at D18S51

 

10.1 [6]

271.83

ABI 3100

ID

Angela Withrow

M U F S C

 

Re-amplified

AL Dept  of Forensic Science Convicted Offender

9 in 14848

10.1 [7]

272.78 +/- 1.42

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

3 in 11500

10.0 [8]

273.32±0.13

ABI 3100

CO/PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

3 on 3750

10.3

231.68

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

colorectal adenocarcinoma (trypsinized variant of LS 180), ATCC cell line CL-188; name:  LS 174T

 

10.3 [2]

232.93

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

prostate carcinoma-from same patient as LNCaP clone FGC, ATCC cell line CRL-10995; name:  LNCaP-FGC-10

 

10.3 [3]

232.96

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

prostate carcinoma (metastatic site:  left supraclavicular lymph

node), ATCC cell line CRL-1740; name:  LNCaP clone FGC [also appears as

LNCaP.FGC]

 

10.3 [4]

275.47

ABI 310

PS

Jeff Sailus

 

 

 

10.3 [5]

277.70

ABI 310

PR, MP

Margaret Kline

Reamplified and verified on a gel

 

 

10.3 [6]

275.38

ABI 310

PS, CO

Robert Sailors

Observed with kit overlap

 

 

10.3 [7]

278.01

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in 1959

10.3 [8]

274.95

ABI 310

PS,CO

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660
10.3 [9] 274.36 ABI 310 ID Johnson_E  USACIL Re-extracted and re-amplified Reference Blood  
10.3 [10] 275.01 ABI 3100 ID Jennifer Rossman, Houston Police Department Crime Lab Re-amplified and Re-injected   1
10.3 9 [11] 275.11 (PP)

274.95 (CO)

 ABI 3100 PS,CO Nicole Hahn                M U F S C Seen at overlapping loci, therefore verified 18776 1

11.1

279.80

ABI 377

CO, PS

Nicole Swinton

Re-extracted and re-amplified

 

 

11.1 [2]

235.04

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and Re-amplified

submitted as an ovarian adenocarcinoma, name:  MDAH-2774.  This cell

line material appears to be a mixture-it has six PP loci showing at least a

trisomy

 

11.1 [3]

277.04

ABI 3100

PS & CO

Misty Marra

M U F S C

Amplification x 2

WV Convicted Offender sample

1 in 12432

11.1 [4]

277.59-277.71

ABI 310

PR,CO

Tamara A. Camp, DuPage County Sheriff Crime Lab, Wheaton, IL

Several different amps: PR and CO or a crime scene stain and a standard

 

 

11.1 [5]

276.47 +/- 0.27

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

6 in 11500

11.1 [6]

277.36±0.12

ABI 3100

CO/PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

 

2 in 3750

11.1 [7]

277.49

ABI 3100

CO/PS

Jan Nicklas,VT Forensic Laboratory

Re-amp

Shoulder at ll

 

11.1 [8]

276.27

232.28

ABI 3130

ABI 3130

ID

PP 16

Cepil O., Cherpin NM., Vandenberghe A., Laboratoire Merieux

France

Re-amplified

 

2 in 1200

11.1 [9]

276.37

232.29

ABI 3130

ABI 3130

ID

PP16

Cepil O., Cherpin NM., Vandenberghe A., Laboratoire Merieux

France

Re-amplified

 

2 in 1200

11.3

279.7

ABI 310

PS, CO

Kathy Sartor

Observed with kit overlap

CSO sample

1 in 600

11.3 [2]

235.47

ABI 310

PP 1.2

 Robyn Hedges

ATCC

Re-extracted and re-amplified

acute T cell leukemia; T lymphocyte; transfected with ZAP-70

expression plasmid; name:  P116.cl39 [also appears as "P116.c39"]

 

11.3 [3]

235.82

ABI 310

PP 1.2

Robyn Hedges

ATCC

Re-extracted and re-amplified

cell line J.gamma1.WT

 

11.3 [4]

236.89

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

emergent allele from prostate carcinoma-from same patient as LNCaP

clone FGC, ATCC cell line CRL-10995; name:  LNCaP-FGC-10

 

11.3 [5]

236.02

ABI 310

ABI 377

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

ileocecal colorectal adenocarcinoma, ATCC cell line CCL-244; name:

HCT-8 [also appears as HRT-18]

 

11.3 [6]

281.89

ABI 377

PS

Catherine Allor

Re-amplified and Re-analyzed

Paternity samples only

1 in 16650

11.3 [7]

278.21

ABI 3100

ID

Angela Withrow

M U F S C

 

Re-amplified

 

1 in 11,512

11.3 [8]

279.26

ABI 310

PS & CO

Jennifer Francis, LAPD Crime Lab

Observed with kit overlap

Questioned sample, sperm fraction

 
11.3 [9] 279.48 ABI 310 PS/CO Ryan Paulsen, Illinois State Police Forensic Science CEnter at Chicago Multiple samples, kit overlap Victim standard and unknown sample  
11.3 [10] 279.23 ABI 3100 PS Nicole Hahn     M U F S C Previously seen - no verification 18251 2

12.1

281.54

ABI 310

PS

John Donahue

Reamplified, analyzed twice on ABI 310

 

 

12.1 [2]

239.04

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

:  submitted as an ovarian adenocarcinoma, name:  MDAH-2774.  This cell

line material appears to be a mixture-it has six PP loci showing at least a

trisomy

 

12.1 [3]

281.65

ABI 310

PR

Kelly Duffy/R.Rubocki

 

 

 

12.1 [4]

281.50

ABI 310

PR

Gintautas Svilpa

Observed both from suspect and crime scene stain

 

1

12.1 [5]

283.85

ABI 377

PS

Catherine Allor

Reamplified and Reanalyzed

Paternity samples only

1 in 11100

12.1 [6]

237.34

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

12.1 [7]

280.57

ABI 3130XL

ID

Arnaud Gleizes & Laurent Pene, Laboratoire de Police Scientifique de Lyon, FRANCE

Re-amplified and re-analyzed

 

1 in 11500
12.1 [8] 280.2 ABI 310 ID Overson_T USACIL Re-extracted and re-amplified Reference Blood  

12.2

238.16 1673 RFU

ABI 3100

PP 16

Kelly Carcaise IL State Police DNA Indexing Lab

Re-amplification

Convicted Offender

1

12.3

285.43

ABI 377

CO

Kelly Solis

Texas DPS

Re-extraction

Convicted offender

1 in 68000

13.1

286.8

ABI 310

PR, MP

Margaret Kline

Reamplified with two kits.

 

1 in 600 samples

13.1 [2]

287.58

ABI 377

CO, PS

Nicole Swinton

Re-extracted and Reamplified

 

 

13.1 [3]

241.25

ABI 310

PP 16

Jackie Johnson

MO State Highway Patrol

Re-extract and re-amplify

Convicted Offender

1 in 32660

14.1

246.96

ABI 310

PP 1.2

Greg Sykes

ATCC

Re-extracted and re-amplified

submitted as an isolate from the bone marrow of a patient who was

diagnosed as Stage 4 neuroblastoma (metastases to the bone marrow), name:

AGF.  This cell line material appears to be a mixture-it has four PP loci

showing at least a trisomy

 

14.1 [2]

245.44

ABI 310

PP 16

Malena B. Jimenez, MO State Highway Patrol

Re-extract & Re-amplify

Convicted Offender

1 in 69600

15

248.12

ABI 310

PP 16

Susie J. Odogba

WI State Crime Laboratory - Madison

Re-amplified using PS amplification kit and reanalyzed

question and known samples

1 in 3,666

16

253.16

ABI 310

PP1.2 & MP

Greg Sykes & Jennifer Kallenborn

ATCC

Re-extracted and re-amplified

From cecum (colorectal carcinoma) parental cell line and derived line.ATCC cell line CCL-250 name: SNU-C2B; and derivative ATCC cell line CCL-250.1 name: SNU-C2A

 

24 reported variants as of  12/28/2006

 

Tri-Allelic Patterns

Allele Designation

Instrument

Amp Kit*

Contributor

Verification/Conformation Method(s)

Notes

Frequency

8, 8.1, 10

ABI 3130

CO & PS

Maria Lam

FDLE, Jacksonville

This pattern showed up on all 7 questioned samples for a single case

 

12

8, 9, 12

ABI 3100

PS & CO

Angela Withrow

M U F S C

Re-extracted X 2

West Virginia Convicted Offender

1 in 9960

8, 10, 11

ABI 377

 CO

Kerstin Montelius, Dept of Forensic Genetics, Sweden

Reanalyzed using PS and CO

Paternity test

1 in 15000

8, 10, 10.1

ABI 3100

ID

Angela Withrow

 M U F S C

 

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender sample

1 in 14544

10, 10.1, 11

ABI 3100

ID

Angela Withrow

 M U F S C

 

Re-amp x 1

AL Dept of Forensic Sciences Convicted Offender sample

1 in 14544

10, 11, 12

ABI 3100

ID

Angela Withrow

 M U F S C

Re-extracted x 1

AL Dept of Forensic Sciences Convicted Offender sample

 1 in 13,945

10, 12, 14

ABI 377

In-house multiplex

Martin Whittle,  Sao Paulo, Brasil

Re-amplification as a monoplex

Observed during routing parentage testing, Brazilian caucasian Seen in child where allele 10 is paternal and 12 is maternal

1 in 29590

7 reported three- banded patterns as of 2/5/2007

 

* Use the following abbreviations for amplification kit names:

  • CO: PE Applied Biosystems COfilerTM
  • PR: PE Applied Biosystems ProfilerTM
  • PS: PE Applied Biosystems Profiler PlusTM
  • CV: Promega CTTVTM
  • CA: Promega CTTATM
  • PP: Promega PowerPlexTM
  • MP: Promega MonoPlexTM
  • ID: PE Applied Biosystems IdentifilerTM
  • Other: Please specify

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