D3S1358

Repeat: [AGAT], [TCTA]= bottom strand
 

Reported Primers	Ref.	PCR Primer Sequences
Set 1	148, 502	5'-ACT GCA GTC CAA TCT GGG T-3' (AGAT strand) 5'-ATG AAA TCA ACA GAG GCT TG-3' (TCTA strand)
Set 2	ABI	AmpFlSTR® Profiler Plus™
Set 3	Promega	GenePrint® PowerPlex™ 2.1

PCR Product Sizes of Observed Alleles
 

Allele       (Repeat #)	Set 1,3	Set 2	Repeat Structure	Ref.
8	99 bp	97 bp		variant allele
9	103 bp	101 bp		SGM Plus
10	107 bp	105 bp		SGM Plus
11	111 bp	109 bp		SGM Plus
12	115 bp	113 bp		SGM Plus
13	119 bp	117 bp	TCTA[TCTG]2[TCTA]10	729
14	123 bp	121 bp	TCTA[TCTG]2[TCTA]11	668
15	127 bp	125 bp	TCTA[TCTG]3[TCTA]11	668
15'	127 bp	125 bp	TCTA[TCTG]2[TCTA]12
15.2	129 bp	127 bp		SGM Plus
16	131 bp	129 bp	TCTA[TCTG]3[TCTA]12	668
16'	131 bp	129 bp	TCTA[TCTG]2[TCTA]13	729
16.2	133 bp	131 bp		642
17	135 bp	133 bp	TCTA[TCTG]3[TCTA]13	668
17'	135 bp	133 bp	TCTA[TCTG]2[TCTA]14	729
17.1	136 bp	134 bp		SGM Plus
18	139 bp	137 bp	TCTA[TCTG]3[TCTA]14	668
18.3	142 bp	140 bp		variant allele
19	143 bp	141 bp	TCTA[TCTG]3[TCTA]15	729
20	147 bp	145 bp		729

Allelic Ladders: Commercially available from Applied Biosystems (ladder allele range: 12-19)

Common Multiplexes: AmpFlSTR Blue, PwerPlex 1.1, Profiler, Profiler Plus, and COfiler
 
 

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