NOW AVAILABLE: see http://www.nist.gov/srm
https://srmors.nist.gov/view_detail.cfm?srm=2395
The Certificate of Analysis for SRM 2395 has been updated with additional information for the 5 male components. These samples now have sequence information at over 40 different Y-STR markers and typed at an additional 3 Y-STRs and 42 Y-SNPs. This SRM may be used for calibrating methods for Y-STR typing with any primer set or commercially available kit.
[Supporting Sequence Information] [Yfiler Stutter Percentages] [Stability Information] [PCR and Sequencing Primers Used]
[Original
SRM
2395 Certificate]
Y-chromosome loci present in the following kits are covered by SRM 2395:
PowerPlex Y, Yfiler, Marligen's Signet™ Y-SNP Identification System
Variable SNPs observed in 42 Y-SNP loci examined
| SRM 2395 | AMEL | M207 | M45 | P25 | M89 | DYS391 | M2 | M170 | M172 | M201 | SNP Haplogroup |
| (A/G) | (G/A) | (C/A) | (C/T) | (C/G) | (A/G) | (A/C) | (T/G) | (G/T) | |||
| Component A | XY | G | A | A | T | C | A | A | T | G | R1b |
| Component B | XY | A | G | C | T | C | A | A | G | G | J2 |
| Component C | XY | A | G | C | C | G | G | A | T | G | E3a |
| Component D | XY | A | G | C | T | C | A | A | T | T | G |
| Component E | XY | A | G | C | T | C | A | C | T | G | I |
| Component F | XX | -- |
Original Set of Sequenced Y-STR Loci
DYS19, DYS385 a/b, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS426, DYS435, DYS436, DYS437, DYS438, DYS439, DYS447, DYS448, DYS460, DYS461, DYS462, GATA-H4
Additional Y-STRs with Typing Results
DYS450, DYS456, DYS458, DYS464 a/b/c/d, YCAII a/b
Added Since SRM 2395 Release
DYS635 (GATA-C4)
Component A - Male Genomic DNA 1 (23 repeats)
(TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)9
Component B – Male Genomic DNA 2 (21 repeats)
(TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)11
Component C – Male Genomic DNA 3 (23 repeats)
(TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)13
Component D – Male Genomic DNA 4 (21 repeats)
(TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)11
Component E – Male Genomic DNA 5 (21 repeats)
(TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)11
Stutter percentages for the 5 male SRM 2395 components with the 17 Yfiler loci
|
Sample Name |
A |
B |
C |
D |
E |
|
DYS456 |
9.0 |
9.4 |
10.0 |
9.2 |
9.2 |
|
DYS389I |
5.0 |
5.1 |
6.0 |
4.3 |
6.8 |
|
DYS390 |
9.5 |
7.9 |
3.4 |
5.9 |
8.2 |
|
DYS389II |
10.7 |
9.2 |
10.9 |
9.2 |
11.9 |
|
DYS458 |
7.2 |
6.5 |
8.5 |
7.2 |
7.7 |
|
DYS19 |
5.7 |
6.4 |
7.7 |
6.9 |
9.1 |
|
DYS385a |
8.4 |
8.2 |
9.8 |
8.3 |
7.4 |
|
DYS385b |
9.2 |
14.7 |
14.8 |
N/A* |
10.1 |
|
DYS393 |
9.5 |
7.4 |
8.0 |
8.5 |
8.8 |
|
DYS391 |
6.6 |
6.8 |
7.8 |
5.8 |
4.3 |
|
DYS439 |
7.5 |
5.7 |
5.5 |
5.8 |
5.0 |
|
DYS635 |
4.6 |
6.6 |
8.5 |
7.1 |
6.8 |
|
DYS392 |
12.1 |
7.5 |
8.6 |
7.1 |
8.8 |
|
DYS392 (N+3) |
6.4 |
3.4 |
3.2 |
3.1 |
3.2 |
|
Y-GATA-H4 |
7.0 |
6.6 |
6.5 |
6.5 |
6.1 |
|
DYS437 |
4.5 |
3.6 |
3.6 |
5.5 |
5.5 |
|
DYS438 |
2.5 |
1.9 |
2.1 |
2.2 |
2.5 |
|
DYS448 |
2.2 |
2.6 |
2.4 |
2.3 |
2.5 |
*N/A – stutter could not be determined due to allele in stutter position
Supporting Sequence Information
Certified allele repeat numbers using GS500-LIZ size standard for base pair sizing
|
|
DYS456 |
Repeat Motif |
bp size |
|
|
DYS607 |
Repeat Motif |
bp size |
|
A |
15 |
[AGAT]15 |
111.15 |
|
A |
19 |
[GAAG]15[GAAA][GAAG][GAAA][GAAG] |
198.16 |
|
B |
15 |
[AGAT]15 |
111.13 |
|
B |
19 |
[GAAG]15[GAAA][GAAG][GAAA][GAAG] |
197.86 |
|
C |
15 |
[AGAT]15 |
111.14 |
|
C |
19 |
[GAAG]15[GAAA][GAAG][GAAA][GAAG] |
197.94 |
|
D |
15 |
[AGAT]15 |
111.13 |
|
D |
17 |
[GAAG]13[GAAA][GAAG][GAAA][GAAG] |
190.12 |
|
E |
15 |
[AGAT]15 |
110.97 |
|
E |
18 |
[GAAG]14[GAAA][GAAG][GAAA][GAAG] |
194.02 |
|
|
|
|
|
|
|
|
|
|
|
|
DYS458 |
Repeat Motif |
bp size |
|
|
DYS635 |
Repeat Motif |
bp size |
|
A |
16 |
[GAAA]16 |
140.39 |
|
A |
23 |
[TCTA]4[TGTA]2[TCTA]2[TGTA]2[TCTA]2[TGTA]2[TCTA]9 |
260.32 |
|
B |
15 |
[GAAA]14 GGAA |
136.52 |
|
B |
21 |
[TCTA]4[TGTA]2[TCTA]2[TGTA]2[TCTA]11 |
252.66 |
|
C |
17 |
[GAAA]17 |
144.69 |
|
C |
23 |
[TCTA]4[TGTA]2[TCTA]2[TGTA]2[TCTA]13 |
260.39 |
|
D |
16 |
[GAAA]16 |
140.36 |
|
D |
21 |
[TCTA]4[TGTA]2[TCTA]2[TGTA]2[TCTA]11 |
252.17 |
|
E |
16 |
[GAAA]16 |
140.31 |
|
E |
21 |
[TCTA]4[TGTA]2[TCTA]2[TGTA]2[TCTA]11 |
252.05 |
|
|
|
|
|
|
|
|
|
|
|
|
DYS492 |
Repeat Motif |
bp size |
|
|
DYS652 |
Repeat Motif |
bp size |
|
A |
12 |
[ATT]12 |
217.81 |
|
A |
24 |
[TAATA]6 TAAAA [TAATA]10 TAAAA [TAATA]6 |
210.76 |
|
B |
12 |
[ATT]12 |
217.86 |
|
B |
25 |
[TAATA]9 TAAAA [TAATA]8 TAAAA [TAATA]6 |
215.87 |
|
C |
11 |
[ATT]11 |
214.64 |
|
C |
25 |
[TAATA]7 TAAAA [TAATA]8 TAAAA [TAATA]8 |
215.81 |
|
D |
11 |
[ATT]11 |
214.57 |
|
D |
23 |
[TAATA]6 TAAAA [TAATA]9 TAAAA [TAATA]6 |
205.64 |
|
E |
12 |
[ATT]12 |
217.67 |
|
E |
26 |
[TAATA]7 TAAAA [TAATA]11 TAAAA [TAATA]6 |
220.61 |
|
|
|
|
|
|
|
|
|
|
|
|
DYS532 |
Repeat Motif |
bp size |
|
|
DYS709 |
Repeat Motif |
bp size |
|
A |
15 |
[CTTT]15 |
490.63 |
|
A |
13 |
[CTTT]13 |
197.83 |
|
B |
11 |
[CTTT]11 |
474.47 |
|
B |
16 |
[CTTT]16 |
209.96 |
|
C |
12 |
[CTTT]12 |
478.52 |
|
C |
15 |
[CTTT]15 |
205.89 |
|
D |
15 |
[CTTT]15 |
490.38 |
|
D |
17 |
[CTTT]17 |
213.75 |
|
E |
9 |
[CTTT]9 |
466.35 |
|
E |
16 |
[CTTT]16 |
209.76 |
|
|
|
|
|
|
|
|
|
|
|
|
DYS534 |
Repeat Motif</ |