SRM 2391b PCR

SRM 2391b PCR-based DNA Profiling Standard

NOW AVAILABLE: see http://www.nist.gov/srm

https://www-s.nist.gov/srmors/view_detail.cfm?srm=2391B

The Certificate of Analysis for SRM 2391b has been updated to include information from 26 new STR loci as well as the MiniFiler kit.

For further information on the new STR loci, see Hill, C.R., Kline, M.C., Coble, M.D., Butler, J.M. (2008) Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples. J. Forensic Sci. 53(1):73-80.

[Supporting Sequence Information] [Stability Information] [MiniFiler Information] [CSF1PO 9948 Tri-Allelic Pattern]

[Certificate Available: https://srmors.nist.gov/view_cert.cfm?srm=2391b]

Supporting Sequence Information

Allele assignments with associated repeat motifs established by sequencing are considered Certified Values. Those allele assignments without sequence established repeat motifs are considered Reference Values.

		D4S2364			D10S1248			D14S1434
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	9	[ATTC]₇ ATCC ATTC	75.33	14	[GGAA]₁₄	103.51	13	[CTGT]₃ [CTAT]₁₀	86.28
				16	[GGAA]₁₆	111.34	14	[CTGT]₃ [CTAT]₁₁	90.49

2	9	[ATTC]₇ ATCC ATTC	75.33	13	[GGAA]₁₃	99.52	11	[CTGT]₃ [CTAT]₈	77.96
	10	[ATTC]₈ ATCC ATTC	79.38	15	[GGAA]₁₅	107.38	13	[CTGT]₃ [CTAT]₁₀	86.23

3	9	[ATTC]₇ ATCC ATTC	75.33	13	[GGAA]₁₃	99.52	14	[CTGT]₃ [CTAT]₁₁	90.42
	10	[ATTC]₈ ATCC ATTC	79.41	16	[GGAA]₁₆	111.30	15	[CTGT]₃ [CTAT]₁₂	94.64

4	9	[ATTC]₇ ATCC ATTC	75.34	12	[GGAA]₁₂	95.31	10	[CTGT]₃ [CTAT]₇	73.84
							11	[CTGT]₃ [CTAT]₈	77.87

5	9	[ATTC]₇ ATCC ATTC	75.30	14	[GGAA]₁₄	103.48	13	[CTGT]₃ [CTAT]₁₀	86.32
	10	[ATTC]₈ ATCC ATTC	79.37	15	[GGAA]₁₅	107.40	14	[CTGT]₃ [CTAT]₁₁	90.51

6	8	[ATTC]₆ ATCC ATTC	71.38	14	[GGAA]₁₄	103.50	13	[CTGT]₃ [CTAT]₁₀	86.29
	9	[ATTC]₇ ATCC ATTC	75.30	15	[GGAA]₁₅	107.38	14	[CTGT]₃ [CTAT]₁₁	90.46

7	9	[ATTC]₇ ATCC ATTC	75.26	13	[GGAA]₁₃	99.56	10	[CTGT]₃ [CTAT]₇	73.90
				14	[GGAA]₁₄	103.51	14	[CTGT]₃ [CTAT]₁₁	90.45

8	9	[ATTC]₇ ATCC ATTC	75.35	11	[GGAA]₁₁	91.17	13	[CTGT]₃ [CTAT]₁₀	86.25
				15	[GGAA]₁₅	107.38

9	9	[ATTC]₇ ATCC ATTC	75.37	13	[GGAA]₁₃	99.58	11	[CTGT]₃ [CTAT]₈	78.04
	10	[ATTC]₈ ATCC ATTC	79.43	15	[GGAA]₁₅	107.43	13	[CTGT]₃ [CTAT]₁₀	86.31

10	9	[ATTC]₇ ATCC ATTC	75.33	12	[GGAA]₁₂	95.37	13	[CTGT]₃ [CTAT]₁₀	86.33
	10	[ATTC]₈ ATCC ATTC	79.38	15	[GGAA]₁₅	107.44	14	[CTGT]₃ [CTAT]₁₁	90.51

11	9		74.94*	13		99.32*	11		77.49*
	10		78.99*	15		107.18*	13		85.68*

12	9		74.94*	12		95.09*	13		85.63*
	10		78.99*	15		107.19*	14		89.81*

		D1S1677			D2S441			D22S1045
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	12	[TTCC]₁₂	89.66	11	[TCTA]₁₁	86.04	14	[ATT]₁₁ ACT [ATT]₂	94.45
	13		93.79	14	[TCTA]₁₁ TTTA [TCTA]₂	98.69	15	[ATT]₁₂ ACT [ATT]₂	97.63

2	14	[TTCC]₁₄	97.99	11	[TCTA]₁₁	86.05	11	[ATT]₈ ACT [ATT]₂	84.89
	16	[TTCC]₁₆	105.97	14	[TCTA]₁₁ TTTA [TCTA]₂	98.69	16		100.68

3	14	[TTCC]₁₄	97.99	10	[TCTA]₈ TCTG TCTA	82.05	15	[ATT]₁₂ ACT [ATT]₂	97.55
	17	[TTCC]₁₇	109.91	14	[TCTA]₁₁ TTTA [TCTA]₂	98.73	16		100.69

4	14	[TTCC]₁₄	97.96	12		90.20	17		103.62
	15	[TTCC]₁₅	102.01	14	[TCTA]₁₁ TTTA [TCTA]₂	98.67	18	[ATT]₁₅ ACT [ATT]₂	106.61

5	13	[TTCC]₁₃	93.71	11	[TCTA]₁₁	85.98	11	[ATT]₈ACT [ATT]₂	85.04
	14	[TTCC]₁₄	97.88	14	[TCTA]₁₁ TTTA [TCTA]₂	98.60	14	[ATT]₁₁ ACT [ATT]₂	94.46

6	13	[TTCC]₁₃	93.67	10	[TCTA]₁₀	81.85	11	[ATT]₈ ACT [ATT]₂	84.98
	14	[TTCC]₁₄	97.86	11	[TCTA]₁₁	85.97	15	[ATT]₁₂ ACT [ATT]₂	97.60

7	12	[TTCC]₁₂	89.53	11	[TCTA]₁₁	85.94	11	[ATT]₈ ACT [ATT]₂	84.95
	13		93.70	14	[TCTA]₁₁ TTTA [TCTA]₂	98.59	15	[ATT]₁₂ ACT [ATT]₂	97.62

8	14	[TTCC]₁₄	98.00	11		86.09	16	[ATT]₁₃ ACT [ATT]₂	100.75
	16	[TTCC]₁₆	105.97	11.3	[TCTA]₄ TCA [TCTA]₇	89.14	17	[ATT]₁₄ ACT [ATT]₂	103.72

9	13	[TTCC]₁₃	93.85	10	[TCTA]₈ TCTG TCTA	82.12	11	[ATT]₈ ACT [ATT]₂	84.97
	14	[TTCC]₁₄	98.03	14	[TCTA]₁₁ TTTA [TCTA]₂	98.76	14	[ATT]₁₁ ACT [ATT]₂	94.47

10	13	[TTCC]₁₃	93.80	11	[TCTA]₁₁	86.06	16		100.81
	14	[TTCC]₁₄	98.02	12	[TCTA]₁₂	90.12	18	[ATT]₁₅ ACT [ATT]₂	106.75

11	13		93.22*	10		81.61*	11		84.42*
	14		97.39*	14		98.13*	14		93.82*

12	13		93.24*	11		85.56*	16		100.07*
	14		97.46*	12		89.74*	18		106.05*

		D1GATA113			D1S1627			D2S1776			D3S3053
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	11	[GATA]₁₁	97.57	10	[ATT]₁₀	81.16	11		144.33	9		92.89
				14	[ATT]₁₄	93.68	12		148.79	12		105.18

2	12		101.66	13		90.56	11	[AGAT]₁₁	144.34	10		97.32
	13		105.58	14		93.71				11		101.48

3	11	[GATA]₁₁	97.51	13		90.52	8		131.54	9		92.93
				14		93.70	10		139.86	11		101.30

4	13		105.55	11		84.27	11		144.34	11	[TATC]₁₁	101.31
				12		87.39	12		148.75

5	11		97.56	14		93.69	12		148.76	11	[TATC]₁₁	101.31
	12		101.64	15		96.83	13		153.09

6	11		97.58	11		84.22	11		144.37	9	[TATC]₉	92.94
	12		101.71	13		90.50	12		148.81

7	10		93.37	11	[ATT]₁₁	84.23	11		144.35	11	[TATC]₁₁	101.29
	12		101.68	14	[ATT]₁₄	93.66	12		148.76

8	10		93.37	13		90.51	11		144.37	9	[TATC]₉	92.87
	12		101.68	14		93.68	12		148.80

9	11		97.51	13	[ATT]₁₃	90.54	10	[AGAT]₁₀	139.88	9		92.96
	12		101.63	14	[ATT]₁₄	93.71				11		101.32

10	7		80.92	11	[ATT]₁₁	84.24	10		139.90	9		92.91
	12		101.73	13	[ATT]₁₃	90.50	12		148.81	12		105.22

11	11		97.06*	13		89.80*	10	[AGAT]₁₀	139.55*	9		92.40*
	12		101.20*	14		92.91*				11		100.80*

12	7		80.47*	11		83.59*	10		139.64*	9		92.41*
	12		101.21*	13		89.80*	12		148.50*	12		104.73*

		D3S4529			D4S2408			D5S2500
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	14		123.24	10	[ATCT]₁₀	97.31	17		97.47
	15		127.37				18		101.47

2	13		199.17	9	[ATCT]₉	93.07	17	[GGTA]₃ [GACA]₈ [GATA]₃ [GATT]₃	97.46
	16		131.42				24	[GGTA]₁₀ [GACA]₆ [GATA]₃ [GATT]₅	126.29

3	14		123.24	8		88.87	17		97.44
	16		131.45	9		93.05	18		101.47

4	15		127.45	9		93.05	17		97.41
	16		131.56	10		97.23	18		101.41

5	13		119.29	10		97.29	14		85.04
	15		127.46	11		101.43	15		89.09

6	15		127.4	9	[ATCT]₉	93.12	14	[GGTA]₃ [GACA]₆ [GATA]₂ [GATT]₃	85.06
	17		135.66				18	[GGTA]₃ [GACA]₉ [GATA]₃ [GATT]₃	101.47

7	14		123.32	8		88.95	14	[GGTA]₃ [GACA]₆ [GATA]₂ [GATT]₃	85.04
	16		131.49	11		101.47	20	[GGTA]₈ [GACA]₅ [GATA]₃ [GATT]₄	110.15

8	14	[ATCT]₉ ATTT [ATCT]₄	123.32	11	[ATCT]₁₁	101.47	14		85.08
							18		101.46

9	13	[ATCT]₈ ATTT [ATCT]₄	119.31	9		93.04	14	[GGTA]₃ [GACA]₆ [GATA]₂ [GATT]₃	85.06
				10		97.24	23	[GGTA]₉ [GACA]₆ [GATA]₃ [GATT]₅	121.14

10	12	[ATCT]₇ ATTT [ATCT]₄	115.24	10	[ATCT]₁₀	97.43	14	[GGTA]₄ [GACA]₆ [GATA]₁ [GATT]₃	85.35
							17	[GGTA]₄ [GACA]₇ [GATA]₃ [GATT]₃	97.40

11	13	[ATCT]₈ ATTT [ATCT]₄	118.47*	9		92.56*	14		84.69*
				10		96.76*	23		121.85*

12	12	[ATCT]₇ ATTT [ATCT]₄	114.51*	10	[ATCT]₁₀	96.72*	14		84.72*
							17		97.05*

		D6S474			D6S1017			D8S1115			D9S1122
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	15		116.12	10	[ATCC]₁₀	93.66	16	[ATT]₁₆	83.32	11		101.27
	17		124.17							12		105.21

2	14		112.26	10		93.64	16	[ATT]₁₆	83.29	12		105.27
	17		124.35	12		101.80				13		109.22

3	14		112.23	10		93.61	16		83.30	12	TAGA TCGA [TAGA]₁₀	105.24
	15		116.21	12		101.80	17		86.44

4	14		112.21	7		81.35	9		62.34	12	TAGA TCGA [TAGA]₁₀	105.20
	16		120.19	10		93.61	17		86.46

5	15		116.23	8		85.39	9		62.35	11		101.28
	18		128.32	9		89.49	15		80.20	13		109.12

6	14		112.21	10	[ATCC]₁₀	93.60	9		62.32	11		101.29
	17		124.29				16		83.34	12		105.20

7	15		116.19	7		81.38	9		62.32	11		101.34
	17		124.26	12		101.76	18		89.63	12		105.25

8	17	[AGAT]₅ [GATA]₁₂	124.20	10		93.64	15		80.23	13	TAGA TCGA [TAGA]₁₁	109.19
				12		101.79	16		83.32

9	14		112.21	9		89.49	9		62.33	12		105.22
	18		128.33	10		93.61	18		89.61	13		109.20

10	17	[AGAT]₅ [GATA]₁₂	124.28	8	[ATCC]₈	85.52	15		80.21	12		105.26
							17		86.50	15		117.21

11	14		111.93*	9		88.87*	9		62.24*	12		104.97*
	18		128.19*	10		92.91*	18		88.92*	13		108.91*

12	17	[AGAT]₅ [GATA]₁₂	124.06*	8	[ATCC]₈	84.86*	15		79.58*	12		104.97*
							17		85.83*	15		116.99*

		D9S2157			D10S1435			D11S4463			D12ATA63
	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size	Type	Repeat motif	Size
1	8		73.46	13	[TATC]13	114.83	14	[TATC]₁₂ TATG TATC	103.77	14		88.00
	13		88.78							17		97.40

2	9		76.47	11		106.83	13		99.91	13		84.89
	11		82.60	14		118.81	14		103.75	17		97.37

3	11		82.60	13		114.79	14		103.88	12		81.83
	13		88.77	14		118.81	15		107.75	15		91.04

4	11	[ATA]₁₁	82.65	12	[TATC]₁₂	110.80	11		91.47	16		94.22
							12		95.72	18		100.47

5	7		70.60	11		106.75	13		99.87	13		84.89
	14		91.93	12		110.74	15		107.79	15		91.10

6	11		82.61	12	[TATC]₁₂	110.76	15		107.77	14		87.94
	13		88.79				16		111.90	18		100.47

7	12		85.68	12	[TATC]₁₂	110.79	13		99.87	16		94.29
	15		95.08				14		103.93	17		97.43

8	11	[ATA]₁₁	82.62	11		106.80	13		99.87	14		87.99
				13		114.78	16		111.77	15		91.01

9	7		70.61	10		102.84	12		95.80	13	[TAA]₁₀[CAA]₃	84.87
	13		88.77	11		106.80	13		100.04

10	7		70.60	12		110.75	12		95.65	13		84.91
	11		82.61	13		114.88	14		103.95	18		100.53

11	7		70.41*	10		102.17*	12		95.08*	13	[TAA]₁₀[CAA]₃	84.47*
	13		88.21*	11		106.16*	13		99.29*

12	7		70.41*	12		110.21*	12		94.94*	13		84.53*
	11		82.10*	13		114.24*	14		103.31*	18		100.07*

D17S974

D17S1301

D18S853