qPCR Workshop Materials

For workshop held at NFSTC on July 26-27, 2006 by Peter Vallone (NIST) and Cristián Orrego (CA DOJ)

Handouts for PowerPoint presentations:

Introduction

Quantitation Using PCR

Instrumentation

Signals and Probes

Selected Forensic qPCR Assays:

VT Alu SYBR Green and Quantifiler

CA DOJ nuclear/mtDNA duplex and CA DOJ degradation triplex

Maintenance, Validation, etc.

Data Analysis and Troubleshooting

qPCR Analysis Software for ABI 7000 & 7500