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NIST has developed a targeted program addressing the
technical infrastructure (measurement science, standards, data, models)
required to support Gene Expression profiling with microarrays.
Currently, there is need for better understanding of the quality
of microarray results. The inability to establish performance has led
to poor confidence in microarray results, difficulty in assessing the
agreement of different experiments, conflicting reports in the
literature, and lost opportunity.
The goal of the NIST program is to enable
measurements of known quality for microarray gene expression results.
Results of known quality will better support research applications in
bioscience, and will facilitate adoption of microarray gene expression
measurements in regulated applications. Measurements of known quality
will permit microarray results to support pharmaco- and toxico-genomics
results for new drug applications, and will lead to new in-vitro diagnostic (IVD) devices.
The Metrology for Gene Expression program is actively:
- developing approaches and standards for experiment validation, in conjunction with the External RNA Control Consortium
- establishing standard methods for measuring microarray performance
- establishing an approach and tools for validation and performance assessment of microarray scanners
- investigating the energetics of nucleic acid hybridization
- characterizing statistical testing methods for microarray data analysis
- assessing parameters and approaches for spectroscopic detection of hybridized complexes
- studying within-lab, between-lab, and between-platform variability
Highlights
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