Bioprocess characterization is focused on industrially important biotransformation problems such as those found in hydrogenation, hydroxylation and aromatic amino acid metabolic pathways. The methods used include site-directed mutagenesis, circular dichroism, ellipsometry, spectroelectrochemistry, and X-ray diffraction. Biocatalysis studies usually involve a coordinated application of several analytical methods such as spectroscopy/kinetics, thermodynamics, and structure measurements. We have recently applied some or all of these tools to alanine dehydrogenase, putidaredoxin and desaturase to gain structural and mechanistic information, and are currently focusing on the pharmaceutically important class of nitrilase enzymes.
Expertise
- molecular biology
- enzyme kinetics
- spectroscopy
- electrochemistry
- mass spectrometry
- x-ray crystallography
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